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Polygalactan from bivalve Crassostrea madrasensis attenuates nuclear factor- κB activation and cytokine production in lipopolysaccharide-activated macrophage

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Relation http://eprints.cmfri.org.in/14471/
https://www.sciencedirect.com/science/article/pii/S0144861720309905
https://doi.org/10.1016/j.carbpol.2020.116817
 
Title Polygalactan from bivalve Crassostrea madrasensis attenuates nuclear factor-
κB activation and cytokine production in lipopolysaccharide-activated
macrophage
 
Creator Chakraborty, Kajal
Krishnan, Soumya
Joy, Minju
 
Subject Bioactive compounds
Biochemistry
Bivalves
Fish Biotechnology
 
Description A polygalactosamino-glucopyranosyl fucopyranose →4)-β-GlcAp{(3→1)-α-Fucp}-β-GalNAcp-(4,6-SO3−)-(1→ isolated from the bivalve Crassostrea madrasensis exhibited prospective anti-inflammatory activity against cyclooxygenase-2 and 5-lipoxygenase (IC50 < 50 μg mL−1) on lipopolysaccharide-induced macrophages. The polygalactan attenuated inducible nitric oxide synthase (IC50 65.7 μg mL−1) in lipopolysaccharide-prompted inflammation leading to the reduction of pro-inflammatory cytokine nitric oxide (236.2 μg mL−1 lysate), nuclear factor-κB, tumor necrosis factor-α, and interleukins (0.19−0.22 units mg−1 protein at 100 μg mL-1) by inhibiting cyclooxygenase-2. The polygalacatan suppressed the mRNA of nuclear factor-κB and cyclooxygenase-2 in lipopolysaccharide-induced macrophages. Western blot experiment revealed that the polygalactan attenuated the migration of nuclear factor-κB-p65 to the nucleus from cytoplasm, and suppressed the phosphorylation of α-subunit of κB inhibitor. Greater selectivity index of sulfated polygalactan (3.93) towards inducible cyclooxygenase-2 as compared with the anti-inflammatory agent ibuprofen (1.11), and the potential to inhibit nuclear factor-κB cascade to generate chemokine production manifested its utilization in the development of functional food attenuating inflammation-related disorders.
 
Date 2020
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/14471/1/Carbohydrate%20Polymers_2020_Kajal%20Chakraborty_Polygalactan%20from%20bivalve%20Crassostrea%20madrasensis.pdf
Chakraborty, Kajal and Krishnan, Soumya and Joy, Minju (2020) Polygalactan from bivalve Crassostrea madrasensis attenuates nuclear factor- κB activation and cytokine production in lipopolysaccharide-activated macrophage. Carbohydrate Polymers, 249. pp. 1-11.