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n-3 Fatty Acids Abrogate Dyslipidemia-Induced Changes in Bile Acid Uptake, Synthesis, and Transport in Young and Aged Dyslipidemic Rats.

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Relation http://ir.cftri.com/14460/
https://dx.doi.org/10.1002/lipd.12125
 
Title n-3 Fatty Acids Abrogate Dyslipidemia-Induced Changes in Bile
Acid Uptake, Synthesis, and Transport in Young and Aged
Dyslipidemic Rats.
 
Creator Pooja, Acharya
Ramaprasad, T. R.
 
Subject 17 Fatty Acid Chemistry
 
Description In this study, the effect of n-3 fatty acids
(FA) [α-linolenic acid (ALA) and eicosapentaenoic acid
(EPA) + docosahexaenoic acid (DHA)] on the intestinal bile
acid (BA) uptake, hepatic BA synthesis, and enterohepatic
bile acid transporters (BAT) was assessed in young and aged
dyslipidemic rats. Dyslipidemia was induced in young and
aged rats by feeding a high-fat (HF) diet. Experimental
groups received diets containing canola oil (HF + CNO) and
fish oil (HF + FO) as a source of ALA and EPA + DHA,
respectively. After 60 days of feeding, intestinal BA uptake
and expression of apical sodium-dependent bile acid transporter (Asbt), organic solute transporter-alpha/beta (Osta/b)
messenger RNA (mRNA), and hepatic expression of Na+
taurocholate cotransporting polypeptide (Ntcp), bile salt
export pump (Bsep), cholesterol 7-α hydroxylase A1
(Cyp7a1), Farnesoid X receptor (Fxr), small heterodimer
partner-1 (Shp), liver receptor homolog-1 (Lrh-1), and
hepatic nuclear factor-4 alpha (Hnf4a) mRNA were measured. Hepatic 3-hydroxy-3-methyl-glutaryl-coenzyme A
(HMG-CoA) reductase activity and total BA in serum, liver,
and feces were assessed. The dyslipidemic HF group had:
(1) increased intestinal BA uptake and Asbt and Osta/b
mRNA expression, (2) increased BA in serum, (3) decreased
hepatic expression of Ntcp, Bsep, and Cyp7a1 mRNA,
(4) increased activity of HMG-CoA reductase, (5) increased
hepatic expression of Fxr and Shp mRNA, (6) decreased
hepatic expression of Lrh-1 and Hnf4a mRNA, and
(7) decreased BA in feces, when compared to control,
HF + CNO, and HF + FO groups. Immunostaining revealed
increased expression of intestinal Asbt and hepatic Ntcp protein in the HF group when compared to control, HF + CNO,
and HF + FO groups. n-3 FA abrogated dyslipidemiainduced changes in the intestinal uptake, hepatic synthesis,
and enterohepatic transporters of BA in both young and aged
rats. EPA + DHA was more effective than ALA in modulating dyslipidemia-induced changes.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14460/1/Lipids%20%282019%29.pdf
Pooja, Acharya and Ramaprasad, T. R. (2019) n-3 Fatty Acids Abrogate Dyslipidemia-Induced Changes in Bile Acid Uptake, Synthesis, and Transport in Young and Aged Dyslipidemic Rats. Lipids. ISSN 0024-4201