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Bio-mining drugs from the sea: High antibiofilm properties of haemocyanin purified from the haemolymph of flower crab Portunus pelagicus (L.) (Decapoda: Portunidae)

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Relation http://eprints.cmfri.org.in/13623/
https://www.sciencedirect.com/science/article/pii/S0044848617316927
https://doi.org/10.1016/j.aquaculture.2018.02.014
 
Title Bio-mining drugs from the sea: High antibiofilm properties of haemocyanin
purified from the haemolymph of flower crab Portunus pelagicus (L.)
(Decapoda: Portunidae)
 
Creator Ishwarya, R
Vaseeharan, B
Jayakumar, R
Ramasubramanian, V
Govindarajan, M
Alharbi, N S
Khaled, J M
Al-anbr, M N
Benelli, Giovanni
 
Subject Bioactive compound
Fish Biotechnology
 
Description Marine organisms are an outstanding source of molecules with wide biological activities. In this research, we
investigated the antimicrobial protein haemocyanin isolated from the haemolymph of flower crab (Portunus
pelagicus) using gel filtration chromatography. Purified P. pelagicus haemocyanin (Pp-Hc) was subjected to SDSPAGE
analysis and the fraction containing Pp-Hc ran as a distinct band, its molecular weight was 78 kDa. The Pp-
Hc was characterized through HPLC, XRD, CD and FTIR analyses. The antibacterial potential of Pp-Hc was
evaluated against five Gram-positive and ten Gram-negative bacteria through the agar well diffusion method,
which clearly revealed the high bactericidal activity of Pp-Hc. Furthermore, Pp-Hc was studied for its antibiofilm
activity against five biofilm-forming Gram-negative bacteria, i.e., Vibrio alginolyticus, Vibrio harveyi, Vibrio
parahaemolyticus, Pseudomonas aeruginosa, and Proteus vulgaris. Confocal laser scanning microscopy (CLSM) and
light microscopy evidenced significant antibiofilm properties of Pp-Hc at 100 μg/ml concentration. Based on
exopolysaccharide (EPS) quantification and cell surface hydrophobicity (CSH) index, the antibiofilm action of
Pp-Hc was linked to its ability to reduce EPS production and increase cell surface hydrophobicity. The pathogen
recognition mechanism of purified Pp-Hc showed a wide spectrum of binding activities, leading to high toxicity
against both Gram-positive and Gram-negative bacterial species. Furthermore, the purified Pp-Hc showed high
antifungal activity against the pathogen Candida albicans. Haemocyanin is not only involved in transporting
oxygen, it has a relevant function in the flower crab immune system. This work adds useful knowledge for
aquaculture concerns dealing with microbial pathogen control. Overall, our results outlined the high potential of
purified Pp-Hc as a starting material to develop new and effective antimicrobial drugs for aquaculture purposes.
1. Introduction
The flower crab, Portunus pelagicus (L.) (Decapoda: Portunidae) is an
edible crab, which inhabits the intertidal zones all over the Indo-Pacific
region, therefore playing a chief role in the marine ecosystems (Lestang
et al., 2003; Kunsook and Dumrongrojwatthana, 2017). It is a vital
species in aquaculture industries. However, in the last few years, crab
farming has been threatened by the increase of infectious diseases due
to bacterial, fungal and viral pathogens (Destoumieux-Garzon et al.,
2001). Current microbiology and parasitology are facing the growing
lack of effective antibiotic drugs. Indeed, antibiotics must be used
prudently to treat humans and animals, since their prolonged overuse
led to the fast development of resistance in targeted organisms (Davies
and Davies, 2010).
 
Publisher Elsevier
 
Date 2019
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/13623/1/Aquaculture_2019_R%20Jayakumar_Portunus%20pelagicus.pdf
Ishwarya, R and Vaseeharan, B and Jayakumar, R and Ramasubramanian, V and Govindarajan, M and Alharbi, N S and Khaled, J M and Al-anbr, M N and Benelli, Giovanni (2019) Bio-mining drugs from the sea: High antibiofilm properties of haemocyanin purified from the haemolymph of flower crab Portunus pelagicus (L.) (Decapoda: Portunidae). Aquaculture, 489. pp. 130-140.