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Superior haplotypes for haplotype‐based breeding for drought tolerance in pigeonpea ( Cajanus cajan L.)

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Relation http://oar.icrisat.org/11570/
https://doi.org/10.1111/pbi.13422
doi:10.1111/pbi.13422
 
Title Superior haplotypes for haplotype‐based breeding for drought tolerance in pigeonpea ( Cajanus cajan L.)
 
Creator Sinha, P
Singh, V K
Saxena, R K
Khan, A W
Abbai, R
Chitikineni, A
Desai, A
Molla, J
Upadhyaya, H D
Kumar, A
Varshney, R K
 
Subject Drought Tolerance
Plant Breeding
Pigeonpea
Genetics and Genomics
 
Description Haplotype-based breeding, a recent promising breeding approach to develop tailor-made crop
varieties, deals with identification of superior haplotypes and their deployment in breeding
programmes. In this context, whole genome re-sequencing data of 292 genotypes from
pigeonpea reference set were mined to identify the superior haplotypes for 10 droughtresponsive
candidate genes. A total of 83, 132 and 60 haplotypes were identified in breeding
lines, landraces and wild species, respectively. Candidate gene-based association analysis of
these 10 genes on a subset of 137 accessions of the pigeonpea reference set revealed 23 strong
marker-trait associations (MTAs) in five genes influencing seven drought-responsive component
traits. Haplo-pheno analysis for the strongly associated genes resulted in the identification of
most promising haplotypes for three genes regulating five component drought traits. The
haplotype C. cajan_23080-H2 for plant weight (PW), fresh weight (FW) and turgid weight (TW),
the haplotype C. cajan_30211-H6 for PW, FW, TW and dry weight (DW), the haplotype
C. cajan_26230-H11 for FW and DW and the haplotype C. cajan_26230-H5 for relative water
content (RWC) were identified as superior haplotypes under drought stress condition.
Furthermore, 17 accessions containing superior haplotypes for three drought-responsive genes
were identified. The identified superior haplotypes and the accessions carrying these superior
haplotypes will be very useful for deploying haplotype-based breeding to develop nextgeneration
tailor-made better drought-responsive pigeonpea cultivars.
 
Publisher Wiley Open Access
 
Date 2020-05
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11570/1/pbi.13422.pdf
Sinha, P and Singh, V K and Saxena, R K and Khan, A W and Abbai, R and Chitikineni, A and Desai, A and Molla, J and Upadhyaya, H D and Kumar, A and Varshney, R K (2020) Superior haplotypes for haplotype‐based breeding for drought tolerance in pigeonpea ( Cajanus cajan L.). Plant Biotechnology Journal (TSI). pp. 1-9. ISSN 1467-7644