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<p style="text-align: left;">Medium Optimization for Xylanase Production by Recombinant <em>Escherichia coli</em> B24</p>

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Title Statement <p style="text-align: left;">Medium Optimization for Xylanase Production by Recombinant <em>Escherichia coli</em> B24</p>
 
Added Entry - Uncontrolled Name Rashid, W B A B; Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81 310, Skudai, Johor, Malaysia
Malek, R Abd; Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81 310, Skudai, Johor, Malaysia
Wadaan, M A; Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia
Elsayed, E A; Chemistry of Natural and Microbial Products Dept., National Research Centre, Dokki, 12 622 Cairo, Egypt
Sukmawati, D ; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negiri Jakarta, Indonesia
El-Enshasy, H A; School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81 310, Skudai, Johor, Malaysia
 
Uncontrolled Index Term Recombinant; Escherichia coli; Xylanase production; Medium optimization; Fermentation
 
Summary, etc. <p style="text-align: left;">Interest in xylanase enzyme application has led to production of xylanase from recombinant <em>Escherichia coli </em>B24, which is an economic alternative towards higher productivity. Recombinant <em>E. coli</em> used in this study is a ubiquitous bacterium containing xylanase encoding gene from <em>Bacillus halodurans</em>. We investigated xylanase production by recombinant <em>E. coli</em> using classical medium optimization. Six fermentation media had been chosen from literature for xylanase production. Afterwards, the most suitable medium was further optimized by varying the key nutrients. The final optimized cultivation medium consisted of (g.L<sup>-1</sup>): glucose, 2.5; NH<sub>4</sub>Cl, 0.4; KH<sub>2</sub>PO<sub>4</sub>, 3.0; Na<sub>2</sub>HPO<sub>4</sub>, 6.0; MgSO<sub>4</sub>.7H<sub>2</sub>O, 1.0. After medium optimization, maximal volumetric xylanase production (600.25 U.mL<sup>-1</sup>) increased by about 115.22% from the initial un-optimized medium (278.9 U.mL<sup>-1</sup>).</p>
 
Publication, Distribution, Etc. Journal of Scientific and Industrial Research (JSIR)
2020-08-26 14:54:13
 
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http://op.niscair.res.in/index.php/JSIR/article/view/39507
 
Data Source Entry Journal of Scientific and Industrial Research (JSIR); ##issue.vol## 79, ##issue.no## 06
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/JSIR/article/download/39507/0