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OsJAZ9 overexpression modulates jasmonic acid biosynthesis and potassium deficiency responses in rice

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Title OsJAZ9 overexpression modulates jasmonic acid biosynthesis and potassium deficiency responses in rice
 
Creator Singh, Ajit Pal
Pandey, Bipin K.
Mehra, Poonam
Heitz, Thierry
Giri, Jitender
 
Subject Abiotic stress
Potassium defciency
JAZ repressor
Root system architecture
Lateral roots
 
Description Accepted date: 6 August 2020
Potassium (K) represents up to 10% of the plant’s total dry biomass, and its deficiency makes plants highly susceptible to both abiotic and biotic stresses. K shortage results in the inhibition of root and shoots growth, but the underlying mechanism of this response is unclear. Our RNA-Seq and qPCR analysis suggested leading roles for JA pathway genes under K deficiency in rice. Notably, K deficiency and JA application produced similar phenotypic and transcriptional responses. Here, we integrated molecular, physiological and morphological studies to analyze the role of OsJAZ9 in JA homeostasis and K deficiency responses. We raised OsJAZ9 over-expression, knockdown, transcriptional reporter, translational reporter and C-terminal deleted translational reporter lines in rice to establish the role of JA signaling in K ion homeostasis. JA profiling revealed significantly increased JA-Ile levels in OsJAZ9 OE lines under K deficiency. Furthermore, we established that OsJAZ9 overexpression and knockdown result in K deficiency tolerance and sensitivity, respectively, by modulating various K transporters and root system architecture. Our data provide evidence on the crucial roles of OsJAZ9 for improving K deficiency tolerance in rice by altering JA levels and JA responses.
A.P.S., P.M., and B.K.P. acknowledge the
research fellowship by UGC, CSIR, and DBT, respectively. We thank
Dr. Vijayata Singh, CSSRI, Karnal, for help in K and Na estimation.
J.G. acknowledges a grant from the INSA-young scientist project. We
acknowledge the NIPGR metabolomics facility.
 
Date 2020-08-25T06:23:14Z
2020-08-25T06:23:14Z
2020
 
Type Article
 
Identifier Plant Molecular Biology, 104(4-5): 397-410
1573-5028
https://doi.org/10.1007/s11103-020-01047-2
https://link.springer.com/article/10.1007/s11103-020-01047-2
http://223.31.159.10:8080/jspui/handle/123456789/1086
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG