Record Details

Toxicity assessment of <em>Gentiana lutea</em> L. root extract and its monoterpene compounds

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement Toxicity assessment of <em>Gentiana lutea</em> L. root extract and its monoterpene compounds
 
Added Entry - Uncontrolled Name Sobot, Ana Valenta
Savic, Jasmina
Trickovic, Jelena Filipovic
Drakulic, Dunja
Joksic, Gordana
 
Uncontrolled Index Term Gentian root; Gentiopicroside; Loganic acid; Structural alerts; Sweroside; Swertiamarin; Yellow gentian
 
Summary, etc. Root of <em>Gentiana lutea</em> commercially available as gentian root, a natural antidote for different types of poisons, possess antioxidative, immunomodulatory, cytoprotective and anti-inflammatory, and adverse, genotoxic and mutagenic effects. It has monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside as most abundant constituents. In this study, we assessed the toxicity of monoterpenes’ reactive molecular fragments using <em>in silico</em> prediction by VEGA-QSAR platform. Further, we compared the data obtained with <em>in vitro</em> geno- and cyto- toxicity testing of the above monoterpenes and the <em>G. lutea</em> root extract (GE), on human primary unstimulated and mitogen-stimulated peripheral blood mononuclear cells (PBMCs). Viability was assessed by TB and XTT tests after 48 h treatmen. DNA damage was evaluated by alkaline comet assay on unstimulated cells, whereas cytokinesis-block micronucleus assay was employed on mitogen-stimulated PBMCs. Stability of compounds throughout treatment was monitored by UPLC. The observed <em>in vitro</em> results had highest compliance with <em>in silico</em> IRFMN/ISSCAN-CGX prediction model. Compounds showed high stability during experiment while treatment with single compounds reduced number of viable cells and increased DNA damage. GE treatment had toxic impact on unstimulated PBMCs but no significant genotoxic influence on mitogen-stimulated PBMCs. In summary, the mild GE effect suggests that the complexity of crude GE extract chemical composition may attenuate the toxicity of the tested monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside.
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-08-31 00:00:00
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJEB/article/view/39874
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 9 (2020): IJEB [September 2020]
 
Language Note en