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Fine mapping and gene cloning in the post-NGS era: advances and prospects

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Relation http://oar.icrisat.org/11588/
https://doi.org/10.1007/s00122-020-03560-w
doi:10.1007/s00122-020-03560-w
 
Title Fine mapping and gene cloning in the post-NGS era: advances and prospects
 
Creator Jaganathan, D
Bohra, A
Thudi, M
Varshney, R K
 
Subject Plant Breeding
Genetics and Genomics
 
Description Improvement in traits of agronomic importance is the top breeding priority of crop improvement programs. Majority of
these agronomic traits show complex quantitative inheritance. Identification of quantitative trait loci (QTLs) followed by
fine mapping QTLs and cloning of candidate genes/QTLs is central to trait analysis. Advances in genomic technologies
revolutionized our understanding of genetics of complex traits, and genomic regions associated with traits were employed in
marker-assisted breeding or cloning of QTLs/genes. Next-generation sequencing (NGS) technologies have enabled genomewide
methodologies for the development of ultra-high-density genetic linkage maps in different crops, thus allowing placement
of candidate loci within few kbs in genomes. In this review, we compare the marker systems used for fine mapping and
QTL cloning in the pre- and post-NGS era. We then discuss how different NGS platforms in combination with advanced
experimental designs have improved trait analysis and fine mapping. We opine that efficient genotyping/sequencing assays
may circumvent the need for cumbersome procedures that were earlier used for fine mapping. A deeper understanding of
the trait architectures of agricultural significance will be crucial to accelerate crop improvement.
 
Publisher Springer
 
Date 2020-02
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11588/1/s00122-020-03560-w.pdf
Jaganathan, D and Bohra, A and Thudi, M and Varshney, R K (2020) Fine mapping and gene cloning in the post-NGS era: advances and prospects. Theoretical and Applied Genetics (TSI), 133 (5). pp. 1791-1810. ISSN 0040-5752