Record Details

Genome-wide transcriptome and physiological analyses provide new insights into peanut drought response mechanisms

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/11600/
https://doi.org/10.1038/s41598-020-60187-z
doi:10.1038/s41598-020-60187-z
 
Title Genome-wide transcriptome and physiological analyses provide new insights into peanut drought response mechanisms
 
Creator Bhogireddy, S
Xavier, A
Garg, V
Layland, N
Arias, R
Payton, P
Nayak, S N
Pandey, M K
Puppala, N
Varshney, R K
 
Subject Groundnut
Drought
Genetics and Genomics
 
Description Drought is one of the main constraints in peanut production in West Texas and eastern New Mexico
regions due to the depletion of groundwater. A multi-seasonal phenotypic analysis of 10 peanut
genotypes revealed C76-16 (C-76) and Valencia-C (Val-C) as the best and poor performers under deficit
irrigation (DI) in West Texas, respectively. In order to decipher transcriptome changes under DI, RNAseq
was performed in C-76 and Val-C. Approximately 369 million raw reads were generated from 12
different libraries of two genotypes subjected to fully irrigated (FI) and DI conditions, of which ~329
million (90.2%) filtered reads were mapped to the diploid ancestors of peanut. The transcriptome
analysis detected 4,508 differentially expressed genes (DEGs), 1554 genes encoding transcription
factors (TFs) and a total of 514 single nucleotide polymorphisms (SNPs) among the identified DEGs.
The comparative analysis between the two genotypes revealed higher and integral tolerance in C-76
through activation of key genes involved in ABA and sucrose metabolic pathways. Interestingly, one
SNP from the gene coding F-box protein (Araip.3WN1Q) and another SNP from gene coding for the lipid
transfer protein (Aradu.03ENG) showed polymorphism in selected contrasting genotypes. These SNPs
after further validation may be useful for performing early generation selection for selecting drought responsive
genotypes.
 
Publisher Nature Research
 
Date 2020-03
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11600/1/s41598-020-60187-z.pdf
Bhogireddy, S and Xavier, A and Garg, V and Layland, N and Arias, R and Payton, P and Nayak, S N and Pandey, M K and Puppala, N and Varshney, R K (2020) Genome-wide transcriptome and physiological analyses provide new insights into peanut drought response mechanisms. Scientific Reports (TSI), 10 (1). ISSN 2045-2322