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High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea)

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Relation http://oar.icrisat.org/11617/
https://doi.org/10.1007/s00122-020-03594-0
doi:10.1007/s00122-020-03594-0
 
Title High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea)
 
Creator Khan, S A
Chen, H
Deng, Y
Chen, Y
Zhang, C
Cai, T
Ali, N
Mamadou, G
Xie, D
Guo, B
Varshney, R K
Zhuang, W
 
Subject Groundnut
Genetics and Genomics
Plant Disease
Aflatoxins
 
Description Aflatoxin contamination in peanuts caused by Aspergillus flavus is a serious food safety issue for human health
around the world. Host plant resistance to fungal infection and reduction in aflatoxin are crucial for mitigating this problem.
Identification of the resistance-linked markers can be used in marker-assisted breeding for varietal development. Here we
report construction of two high-density genetic linkage maps with 1975 SNP loci and 5022 SNP loci, respectively. Two
consistent quantitative trait loci (QTL) were identified as qRAF-3-1 and qRAF-14-1, which located on chromosomes A03
and B04, respectively. QTL qRAF-3-1 was mapped within 1.67 cM and had more than 19% phenotypic variance explained
(PVE), while qRAF-14-1 was located within 1.34 cM with 5.15% PVE. While comparing with the reference genome, the
mapped QTLs, qRAF-3-1 and qRAF-14-1, were located within a physical distance of 1.44 Megabase pair (Mbp) and 2.22
Mbp, harboring 67 and 137 genes, respectively. Among the identified candidate genes, six genes with the same function were
found within both QTLs regions. In addition, putative disease resistance RPP13-like protein 1 (RPP13), lipoxygenase (Lox),
WRKY transcription factor (WRKY) and cytochrome P450 71B34 genes were also identified. Using microarray analysis,
genes responded to A. flavus infection included coding for RPP13, pentatricopeptide repeat-containing-like protein, and
Lox which may be possible candidate genes for resistance to A. flavus. The QTLs and candidate genes will further facilitate
marker development and validation of genes for deployment in the molecular breeding programs against A. flavus in peanuts.
 
Publisher SPRINGER
 
Date 2020-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11617/1/s00122-020-03594-0-3.pdf
Khan, S A and Chen, H and Deng, Y and Chen, Y and Zhang, C and Cai, T and Ali, N and Mamadou, G and Xie, D and Guo, B and Varshney, R K and Zhuang, W (2020) High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea). Theoretical and Applied Genetics (TSI), 133 (7). pp. 2239-2257. ISSN 0040-5752