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Systemic acquired resistance specifc proteome of Arabidopsis thaliana

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Title Systemic acquired resistance specifc proteome of Arabidopsis thaliana
 
Creator Kumar, Rajiv
Barua, Pragya
Chakraborty, Niranjan
Nandi, Ashis Kumar
 
Subject Comparative proteomic analysis
Diferentially abundant proteins
fld
Plant immunity
rsi1
Systemic acquired resistance
 
Description Accepted date: 20 August 2020
Key message A comparative proteomic study between WT and SAR-compromised rsi1/fd mutant reveals a set of
proteins having possible roles in the SAR development.
Abstract A partly infected plant shows enhanced resistance during subsequent infection through the development of systemic
acquired resistance (SAR). Mobile signals generated at the site of primary infection travel across the plant for the activation
of SAR. These mobile signals are likely to cause changes in the expression of a set of proteins in the distal tissue, which
contributes to the SAR development. However, SAR-specifc proteome is not revealed for any plant. The reduced systemic
immunity 1 (rsi1)/(allelic to fowering locus D; fd) mutant of Arabidopsis is compromised for SAR but shows normal local
resistance. Here we report the SAR-specifc proteome of Arabidopsis by comparing diferentially abundant proteins (DAPs)
between WT and fd mutant. Plants were either mock-treated or SAR-induced by primary pathogen inoculation. For proteomic
analysis, samples were collected from the systemic tissues before and after the secondary inoculation. Protein identifcation
was carried out by using two-dimensional gel electrophoresis (2-DE) followed by tandem mass spectrometry. Our work
identifed a total of 94 DAPs between mock and pathogen treatment in WT and fd mutant. The DAPs were categorized
into diferent functional groups along with their subcellular localization. The majority of DAPs are involved in metabolic
processes and stress response. Among the subcellular compartments, plastids contained the highest number of DAPs, suggesting the importance of plastidic proteins in SAR activation. The fndings of this study would provide resources to engineer
efcient SAR activation traits in Arabidopsis and other plants.
This work was supported by the DBT grant (No. BT/PR23632/BPA/118/312/2017) to AKN and D.S. Kothari post-doctoral fellowship (No. F.4-2/2006 (BSR)/BL/13-14/0456) to RK.
 
Date 2020-09-07T10:15:50Z
2020-09-07T10:15:50Z
2020
 
Type Article
 
Identifier Plant Cell Reports, 39: 1549–1563
1432-203X
https://doi.org/10.1007/s00299-020-02583-3
https://link.springer.com/article/10.1007/s00299-020-02583-3
http://223.31.159.10:8080/jspui/handle/123456789/1092
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG