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Heterotrimeric Gα subunit regulates plant architecture, organ size and seed weight in the oilseed Brassica juncea

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Title Heterotrimeric Gα subunit regulates plant architecture, organ size and seed weight in the oilseed Brassica juncea
 
Creator Kumar, Roshan
Bisht, Naveen C.
 
Subject Heterotrimeric G-protein
Gα subunit
Brassica juncea
Oilseed
Plant architecture
Agronomic traits
 
Description Accepted date: 21 August 2020
Key message Two BjuGα proteins exhibit conserved GTP-binding and GTP-hydrolysis activities, and function in
maintaining overall plant architecture and controlling multiple yield-related traits in the oilseed Brassica juncea.
Abstract Heterotrimeric G-protein (Gα, Gβ and Gγ) are key signal transducers, well characterized in model plants Arabidopsis and rice. However, our knowledge about the roles played by G-proteins in regulating various growth and developmental traits in polyploid crops, having a complex G-protein signalling network, is quite sparse. In the present study, two
Gα encoding genes (BjuA.Gα1 and BjuB.Gα1) were isolated from the allotetraploid Brassica juncea, a globally cultivated
oilseed crop of the Brassicaceae family. BjuGα1 genes share a close evolutionary relationship, and the encoded proteins
exhibit highly conserved G-protein activities while showing expression diferentiation, wherein BjuA.Gα1 was the highly
abundant transcript during plant growth and developmental stages. RNAi based suppression of BjuGα1 displayed compromised efects on most of the tested vegetative and reproductive parameters, particularly plant height (32–58%), fower and
siliques dimensions, and seed weight (11–13%). Further, over-expression of a constitutively active Gα, lacking the GTPase
activity, produced plants with increased height, organ size and seed weight (7–25%), without altering seed quality traits like
fatty acid composition, glucosinolates, oil and protein contents. Our study demonstrates that BjuGα1 proteins control overall
plant architecture and multiple yield-related traits in the oilseed B. juncea, suggesting that BjuGα1 could be a promising
target for crop improvement.
The work was supported by funding from Science and Engineering Board, India (Grant No. EMR/2016/006433) and the core-grant of National Institute of Plant Genome Research (NIPGR) to NCB. RK was funded with the Research Fellowship by the University Grant Commission (India) and NIPGR.
 
Date 2020-09-07T10:34:18Z
2020-09-07T10:34:18Z
2020
 
Type Article
 
Identifier Plant Molecular Biology, 104(4-5): 549-560
1573-5028
https://doi.org/10.1007/s11103-020-01060-5
https://link.springer.com/article/10.1007%2Fs11103-020-01060-5
http://223.31.159.10:8080/jspui/handle/123456789/1093
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG