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Selective adsorption of bovine hemoglobin by Ni2+-functionalized silica nanoparticles
NOPR - NISCAIR Online Periodicals Repository
View Archive Info| Field | Value | |
| Title |
Selective adsorption of bovine hemoglobin by Ni2+-functionalized silica nanoparticles
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| Creator |
Fu, Rao
Jiang, Shan |
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| Subject |
Iminodiacetic acid (IDA)
Protein Bovine hemoglobin (BHb) |
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| Description |
1321-1326
In this work, we have used Stöber method to prepare 200 nm SiO2 particles and then immobilized metal affinity ligand iminodiacetic acid (IDA) and charged them with Ni2+ ions. The particles show high absorption efficiency to bovine hemoglobin and the maximum extraction amounts reached 43.4 mg/g. The particles have been applied to separate a model protein mixture of bovine hemoglobin (BHb) and bovine serum albumin (BSA). They can be separated and showed low non-specific adsorption. Then we have used these particles to extract BHb from the mixture of BHb and DNA. They have potential applications in removing abundant protein in proteomic analysis, and we hope it may become a simple, convenient, and potentially effective way to purify hemoglobin in serum. |
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| Date |
2020-09-26T08:16:36Z
2020-09-26T08:16:36Z 2020-09 |
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| Type |
Article
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| Identifier |
0975-0975(Online); 0376-4710(Print)
http://nopr.niscair.res.in/handle/123456789/55345 |
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| Language |
en_US
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| Rights |
 CC Attribution-Noncommercial-No Derivative Works 2.5 India
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJC-A Vol.59A(09) [September 2020]
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