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Stable release of enhanced organic solvent tolerant amylase from Bacillus amyloliquefaciens AMY02 under sub-merged fermentation

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Title Stable release of enhanced organic solvent tolerant amylase from Bacillus amyloliquefaciens AMY02 under sub-merged fermentation
 
Creator Anbu, Periasamy
Gopinath, Subash CB
So, Jae-Seong
Jayanthi, S
 
Subject Amylase production
Non-aqueous media
Phylogenetic analysis
Solvent-stable enzymes
Solvent tolerant bacteria
 
Description 552-559
This study has been performed to isolate a potential strain able to release the prolific amylase under non-aqueous conditions to meet the current demand in industries to substitute the amylase produced in aqueous media. A bacterial strain that produces organic solvent-stable amylase in the media containing 15% benzene was isolated from the soil. The recovered strain was identified to be Bacillus amyloliquefaciens AMY02by 16S rRNA sequencing. Under sub-merged fermentation, the optimized amylase release by this strain was found with the condition having starch (carbon source), pH 7.0, the temperature at 30°C for 48 h (incubation time). This optimized condition promoted the amylase production to be 2.04-fold higher than the culture was kept under standard condition with the basic media composition. Further, the stability of the enzyme in the presence of 20% organic solvents was assessed by incubating for 2 weeks. The enzyme was found to be active and stable in the presence of benzene, chloroform, o-xylene, and toluene. The higher organic solvent stability of this amylase production by B. amyloliquefaciens under sub-merged fermentation can be an alternative catalyst in non-aqueous media for industrial applications.
 
Date 2020-09-30T05:48:19Z
2020-09-30T05:48:19Z
2020-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/55371
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.57(5) [October 2020]