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Antileishmanial apoptotic activity of Nigella sativa L. essential oil and thymoquinone triggers on Leishmania tropica

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Title Antileishmanial apoptotic activity of Nigella sativa L. essential oil and thymoquinone triggers on Leishmania tropica
 
Creator Al-Turkmani, Mohamad Osama
Mokrani, Loubna
Soukkarieh, Chadi
 
Subject Black Cumin
Cell cycle arrest
Cutaneous leishmaniasis
Programmed cell death
Promastigotes
 
Description 699-705
Nigella sativa L., commonly called Black cumin, is well-known in folk medicine and numerous studies have shown its various pharmacological activities. In this study, we estimated the cytotoxic effects of N. sativa essential oil (NEO) and its major bioactive component Thymoquinone (TQ) on Leishmania tropica promastigotes that cause cutaneous leishmanias, and also observed the programmed cell death features. The extraction of NEO was done by hydro-distillation and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Antileishmanial activity of NEO and TQ was determined using 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) assay and the obtained results are expressed as 50% inhibitory concentration (IC50). The leishmanicidal activity of NEO and TQ was mediated via apoptosis as evidenced by in situ labelling of DNA fragments using terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labelling (TUNEL) and cell cycle arrest at sub G0/G1 phase. The IC50 values were 5 µg/mL and 1.3 µg/mL, respectively. We noted a significant increase in DNA fragmentation in treated parasites with IC50 of both NEO and TQ as well as a cell cycle arrest. These results revealed that NEO and TQ possess potential antileishmanial activity that mediated high possibility by programmed cell death.
 
Date 2020-09-30T09:08:12Z
2020-09-30T09:08:12Z
2020-10
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/55409
 
Language en_US
 
Rights Publisher: NISCAIR-CSIR, India CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJEB Vol.58(10) [October 2020]