Record Details

Amelioration of cisplatin induced nephrotoxicity by Phyla nodiflora (L.) Greene

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Amelioration of cisplatin induced nephrotoxicity by Phyla nodiflora (L.) Greene
 
Creator Sharma, Sonam
Dixit, Priya
Sairam, K
Sahu, Alakh N
 
Subject DNA fragmentation assay
Frog fruit
Jal bhuti
Nephroprotective
Rutin
 
Description 691-698
Phyla nodiflora (Verbenaceae), commonly called frog fruit and locally, jal bhuti, is the traditional folk medicine accepted in the Indian Medicine as well as the Traditional system of Chinese Medicine (TCM) for various treatments viz., urinary disorder, lithiasis, knee joint pain, diuresis and swelling. In the present study, we tried to standardise crude Phyla nodiflora ethanol extract (PNE) using HPTLC and also evaluated its protection against cisplatin induced nephrotoxicity in rats. HPTLC quantification of rutin and fingerprinting profile was performed. Serum kidney toxicity markers, renal tissue antioxidant and pro-inflammatory cytokine levels were assessed followed by DNA fragmentation assay and histopathological examination of renal tissue. Rutin concentration in ethanol extract was found to be 5.35% w/w. In HPTLC fingerprinting 12 peaks with Rf ranges from 0.08 to 0.86 were confirmed. Serum biochemical parameters, renal tissue antioxidants and pro-inflammatory cytokines levels were found to be restored. The results demonstrated the nephroprotective activity of P. nodiflora ethanol extract (400 mg/kg, p.o.) as evidenced by protection of kidneys from cisplatin induced DNA fragmentation and damage tissue architecture.
 
Date 2020-09-30T09:10:32Z
2020-09-30T09:10:32Z
2020-10
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/55410
 
Language en_US
 
Rights Publisher: NISCAIR-CSIR, India CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJEB Vol.58(10) [October 2020]