Record Details

Stable release of enhanced organic solvent tolerant amylase from <em>Bacillus amyloliquefaciens</em> AMY02 under sub-merged fermentation

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement Stable release of enhanced organic solvent tolerant amylase from <em>Bacillus amyloliquefaciens</em> AMY02 under sub-merged fermentation
 
Added Entry - Uncontrolled Name Anbu, Periasamy ; Inha University
Gopinath, Subash CB
So, Jae-Seong
Jayanthi, S
 
Uncontrolled Index Term Amylase production, Non-aqueous media, Phylogenetic analysis, Solvent-stable enzymes, Solvent tolerant bacteria
 
Summary, etc. This study has been performed to isolate a potential strain able to release the prolific amylase under non-aqueous conditions to meet the current demand in industries to substitute the amylase produced in aqueous media. A bacterial strain that produces organic solvent-stable amylase in the media containing 15% benzene was isolated from the soil. The recovered strain was identified to be Bacillus amyloliquefaciens AMY02by 16S rRNA sequencing. Under sub-merged fermentation, the optimized amylase release by this strain was found with the condition having starch (carbon source), pH 7.0, the temperature at 30°C for 48 h (incubation time). This optimized condition promoted the amylase production to be 2.04-fold higher than the culture was kept under standard condition with the basic media composition. Further, the stability of the enzyme in the presence of 20% organic solvents was assessed by incubating for 2 weeks. The enzyme was found to be active and stable in the presence of benzene, chloroform, o-xylene, and toluene. The higher organic solvent stability of this amylase production by B. amyloliquefaciens under sub-merged fermentation can be an alternative catalyst in non-aqueous media for industrial applications.
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2020-09-30 11:41:18
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJBB/article/view/30745
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); IJBB Vol.57(5) [October 2020]
 
Language Note en