Record Details

Effects of verteporfin-mediated photodynamic therapy in breast cancer cells

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement Effects of verteporfin-mediated photodynamic therapy in breast cancer cells
 
Added Entry - Uncontrolled Name Kılıçaslan Sönmez, Pınar
Turhan, Ali
Öztatlıcı, Mustafa
Özbilgin, Kemal
 
Uncontrolled Index Term Bax; Bcl-2; Laser; MDA-MB-231; Photosensitizer
 
Summary, etc. Photodynamic therapy works with a photosensitizer that is stimulated when exposed to a light source of a specific wavelength and produces a form of oxygen that can be used in cancer treatments. In this study, we investigated the effect of laser on apoptosis on breast cancer cell lines (MDA-MB-231) treated with verteporfin in cell culture media. Verteporfin added MDA-MB-231 cells were incubated without light for 24 hours after applying laser light at a wavelength of 695 nm at an intensity of 50 J/cm2 at various times. Anti-proliferative effects were evaluated by immunoreactivity of anti-Bcl-2 and anti-Bax antibodies by immunocytochemical staining. When anti-Bax/Anti-Bcl-2 ratio are compared, the ratio of 1.5 in the control group cells decreases in short-term laser applications, while it approaches normal values in the 7th min after long-term laser application and reaches a very high value in the 9th min. Therefore, our results suggest that verteporfin-mediated PDT may be a potential combined therapy strategy against breast carcinoma by increasing apoptosis.
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2020-09-30 11:41:18
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJBB/article/view/30012
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); IJBB Vol.57(5) [October 2020]
 
Language Note en