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<p><strong>Antileishmanial apoptotic activity of <em>Nigella sativa</em> L. essential oil and thymoquinone triggers on <em>Leishmania tropica</em></strong></p>

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Title Statement <p><strong>Antileishmanial apoptotic activity of <em>Nigella sativa</em> L. essential oil and thymoquinone triggers on <em>Leishmania tropica</em></strong></p>
 
Added Entry - Uncontrolled Name Al-Turkmani, Mohamad Osama
Mokrani, Loubna
Soukkarieh, Chadi
 
Uncontrolled Index Term Black Cumin; Cell cycle arrest; Cutaneous leishmaniasis; Programmed cell death; Promastigotes
 
Summary, etc. <p class="Abstract" style="text-align: justify;"><em>Nigella sativa </em>L., commonly called Black cumin, is well-known in folk medicine and numerous studies have shown its various pharmacological activities. In this study, we estimated the cytotoxic effects of <em>N. sativa</em> essential oil (NEO) and its major bioactive component Thymoquinone (TQ) on <em>Leishmania tropica</em> promastigotes that cause cutaneous leishmanias, and also observed the programmed cell death features. The extraction of NEO was done by hydro-distillation and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Antileishmanial activity of NEO and TQ was determined using 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) assay and the obtained results are expressed as 50% inhibitory concentration (IC<sub>50</sub>). The leishmanicidal activity of NEO and TQ was mediated via apoptosis as evidenced by <em>in situ</em> labelling of DNA fragments using terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labelling (TUNEL) and cell cycle arrest at sub G<sub>0</sub>/G<sub>1</sub> phase. The IC<sub>50</sub> values were 5 µg/mL and 1.3 µg/mL, respectively. We noted a significant increase in DNA fragmentation in treated parasites with IC<sub>50</sub> of both NEO and TQ as well as a cell cycle arrest. These results revealed that NEO and TQ possess potential antileishmanial activity that mediated high possibility by programmed cell death.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-09-30 00:00:00
 
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http://op.niscair.res.in/index.php/IJEB/article/view/41206
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 10 (2020): IJEB [October 2020]
 
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