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Ribosomal protein QM/RPL10 positively regulates defence and protein translation mechanisms during nonhost disease resistance

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Title Ribosomal protein QM/RPL10 positively regulates defence and protein translation mechanisms during nonhost disease resistance
 
Creator Ramu, Vemanna S.
Dawane, Akashata
Lee, Seonghee
Oh, Sunhee
Lee, Hee-Kyung
Sun, Liang
Senthil-Kumar, Muthappa
Mysore, Kirankumar S.
 
Subject disease resistance
plant immunity
ribosome
RNA sequencing
translation regulation
 
Description Accepted date: 19 August 2020
Ribosomes play an integral part in plant growth, development, and defence responses.
We report here the role of ribosomal protein large (RPL) subunit QM/RPL10 in nonhost disease resistance. The RPL10-silenced Nicotiana benthamiana plants showed
compromised disease resistance against nonhost pathogen Pseudomonas syringae
pv. tomato T1. The RNA-sequencing analysis revealed that many genes involved in
defence and protein translation mechanisms were differentially affected due to silencing of NbRPL10. Arabidopsis AtRPL10 RNAi and rpl10 mutant lines showed compromised nonhost disease resistance to P. syringae pv. tomato T1 and P. syringae pv.
tabaci. Overexpression of AtRPL10A in Arabidopsis resulted in reduced susceptibility against host pathogen P. syringae pv. tomato DC3000. RPL10 interacts with the
RNA recognition motif protein and ribosomal proteins RPL30, RPL23, and RPS30 in
the yeast two-hybrid assay. Silencing or mutants of genes encoding these RPL10-
interacting proteins in N. benthamiana or Arabidopsis, respectively, also showed compromised disease resistance to nonhost pathogens. These results suggest that QM/
RPL10 positively regulates the defence and translation-associated genes during nonhost pathogen infection.
This work was supported by the Noble Research Institute, LLC, and
the Ramanujan Fellowship, SERB to V.S.R., and Regional Centre for
Biotechnology core funding. V.S.R. acknowledges a Fulbright-Nehru
postdoctoral fellowship from USIEF and a Ramanujan Fellowship,
SERB, India (SB/S2/RJN-046/2016). K.S.M. acknowledges the
Fulbright-Nehru Academic and Professional Excellence Award.
 
Date 2020-09-30T09:29:32Z
2020-09-30T09:29:32Z
2020
 
Type Article
 
Identifier Molecular Plant Pathology, 21: 1481-1494
1364-3703
https://doi.org/10.1111/mpp.12991
https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12991
http://223.31.159.10:8080/jspui/handle/123456789/1106
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons