Record Details

Cytotoxicity and 3T3-L1 cell uptake of lactucaxanthin purified and characterized by LCMS and NMR from lettuce (Lactuca sativa).

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/14499/
https://doi.org/10.1080/10826076.2020.1719414
 
Title Cytotoxicity and 3T3-L1 cell uptake of
lactucaxanthin purified and characterized by LCMS and NMR from lettuce (Lactuca sativa).
 
Creator Naveen, J.
Anitha Rani, E.
Sachin, R. Chaudhari
Baskaran, V.
 
Subject 17 Toxicology
23 Vegetables
 
Description Lettuce (Lactuca sativa) and its bioactive constituent lactucaxanthin are gaining importance due to
their antidiabetic property. Hence, aim of this study is to establish a simple purification technique
for lactucaxanthin and its antioxidant property, cytotoxicity, and cellular uptake in 3T3-L1 cells
(pre-adipocytes). Results revealed that lactucaxanthin is higher (mg/100 g dry weight) in green lettuce (3.05 ± 0.12) than Romania (0.42 ± 0.05) and Iceberg (0.36 ± 0.06), respectively. The purity of
lactucaxanthin separated by TLC followed by preparatory HPLC is 99 ± 1% with kmax 439 nm. FTIR,
LC-MS (M þ Hþ–H2O ¼ 551) and NMR analysis revealed the characteristic chemical structure of lactucaxanthin. Cytotoxicity of lactucaxanthin in 3T3-L1 cells treated at 1, 3, 5, 10, 30, 50, 60, 100 lM
for 24 hr revealed 86–89% cell viability up to 10 mM concentration. 2,2-Diphenyl-1-picrylhydrazyl
(DPPH), 2,20
-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-mediated radical scavenging
(35.65 ± 0.85 mg/ml and 4.92 ± 0.81 mg/ml), total antioxidant 5 mg (equivalent to 23.71 ± 1.88 ascorbic acid) activity and reductive capability 20 mg (26.14 mg quercetin equivalent) of lactucaxanthin
proved its antioxidant potential. 3T3-L1 cellular uptake of lactucaxanthin was found time dependent with absorption maxima of 46.35 ng after 24 hr treatment (10 mM). The present results help in
effective positioning of lactucaxanthin for food and pharma application to fight against diabetes
and dyslipidemia.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14499/1/Journal%20of%20Liquid%20Chromatography%20%26%20Related%202020.pdf
Naveen, J. and Anitha Rani, E. and Sachin, R. Chaudhari and Baskaran, V. (2020) Cytotoxicity and 3T3-L1 cell uptake of lactucaxanthin purified and characterized by LCMS and NMR from lettuce (Lactuca sativa). Journal of Liquid Chromatography & Related Technologies, 43 (7-8). pp. 233-246.