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Encapsulation of b-amylase in water-oil-water enzyme emulsion liquid membrane (EELM) bioreactor for enzymatic conversion of starch to maltose.

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Relation http://ir.cftri.com/14513/
https://doi.org/10.1080/10826068.2019.1679172
 
Title Encapsulation of b-amylase in water-oil-water enzyme emulsion liquid
membrane (EELM) bioreactor for enzymatic conversion of starch to maltose.
 
Creator Priyanka, B. S.
Navin, K. Rastogi
 
Subject 05 Enzymes
 
Description The b-amylase was encapsulated in emulsion liquid membrane (ELM), which acted as a reactor for
conversion of starch to maltose. The membrane phase was consisted of surfactant (span 80), stabilizer (polystyrene), carrier for maltose transport (methyl cholate) and solvent (xylene). The substrate starch in feed phase entered into the internal phase by the process of diffusion and
hydrolyzed to maltose by encapsulated b-amylase. Methyl cholate present in the membrane acts
as a carrier for the product maltose, which helps in transport of maltose to feed phase from
internal aqueous phase. The residual activity of b-amylase after the five-reaction cycle was found
to decrease to �70%, which indicated possibility to recycle the components of the emulsion and
enzyme. The pH and temperature of the encapsulated enzyme were found to be optimum at 5.5
and 60 C, respectively. The novelty of the present work lies in the development of Enzyme
Emulsion Liquid Membranes (EELM) bioreactor for the hydrolysis of starch into maltose mediated
by encapsulated b-amylase. The attempt has been made for the first time for the successful
encapsulation of b-amylase into EELM. The best results gave the highest residual enzyme activity
(94.1%) and maltose production (29.13 mg/mL).
 
Publisher Taylor & Francis
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14513/1/Preparative%20Biochemistry%20%26%20Biotechnology%2C%20502%2C%20172-180.pdf
Priyanka, B. S. and Navin, K. Rastogi (2020) Encapsulation of b-amylase in water-oil-water enzyme emulsion liquid membrane (EELM) bioreactor for enzymatic conversion of starch to maltose. Preparative Biochemistry and Biotechnology, 50 (2). pp. 172-180. ISSN 1082-6068