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Membrane processing for purification of R-Phycoerythrin from marine macro-alga, Gelidium pusillum and process integration.

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Relation http://ir.cftri.com/14517/
https://doi.org/10.1016/j.seppur.2020.117470
 
Title Membrane processing for purification of R-Phycoerythrin from marine
macro-alga, Gelidium pusillum and process integration.
 
Creator Rochak, M.
Archana, Gopalrao Lamdande
Richa, Sharma
Raghavarao, K. S. M. S.
 
Subject 01 Algae
03 Proteins
 
Description Ultrafiltration is widely used for separation and purification of several biomolecules from various sources.
However, the performance of ultrafiltration in terms of transmembrane flux, yield and purity vary with biomolecule and process parameters. In the present study, ultrafiltration was employed for the purification of RPhycoerythrin from marine macroalga Gelidium pusillum. Parameters such as molecular weight cut-off (30, 50,
100 and 300 kDa), transmembrane pressure (1, 2 and 4 bar), pH (4, 5, 6 and 7), and ionic strength (control, 0.5
and 1.0 M) were standardised. Ultrafiltration in diafiltration mode and its integration with ammonium sulphate
precipitation and aqueous two-phase extraction was also carried out to maximise R-PE purity. In-situ ultrasonication was employed to enhance the transmembrane (permeate) flux. Permeate flux was evaluated employing Hermia’s model to have insight and understand membrane fouling that occurs during ultrafiltration.
Integration of ammonium sulphate precipitation followed by diafiltration and aqueous two-phase extraction has
resulted in 17 fold enrichment in R-PE purity with 61% yield and removal of more than 95% of soluble sugars.
In-situ ultrasonication has resulted in increase of transmembrane flux by 10% to 47 L/m2
/h. Hermia’s model
based analysis of permeate flux has revealed that cake formation is the predominant type of fouling.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14517/1/Separation%20and%20Purification%20Technology%20252%20%282020%29%20117470%20_1.pdf
Rochak, M. and Archana, Gopalrao Lamdande and Richa, Sharma and Raghavarao, K. S. M. S. (2020) Membrane processing for purification of R-Phycoerythrin from marine macro-alga, Gelidium pusillum and process integration. Separation and Purification Technology, 252. p. 117470. ISSN 1383-5866