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<p><strong>Label free biosensor for screening estrogenic activity </strong></p>

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Authentication Code dc
 
Title Statement <p><strong>Label free biosensor for screening estrogenic activity </strong></p>
 
Added Entry - Uncontrolled Name Kumbhat, Sunita ; Jai Narain Vyas University
UGC, EU
 
Uncontrolled Index Term analytical chemistry
surface plasmon resonance; label-free biosensor; estrogenic activity; estrogen mimics; endocrine disrupting chemicals; EDCs
 
Summary, etc. <p>Hormone mimics known as endocrine disrupting compounds (EDCs) prevalent in aquatic environment are of great environmental concern because of their endocrine disrupting and carcinogenic effects. Looking to the wide variety of natural as well as synthetic estrogen hormone mimics, a reliable <em>in-vitro</em> assay is required to screen the estrogenic activity. Surface plasmon resonance (SPR) is one of the most promising analytical tools to monitor high-performance biomolecular interaction in real time without labelling. Present paper demonstrates a facile SPR based affinity bioassay employing estrogen receptor-α, human (hERα) functionalized self assembled monolayer covalently bound to the gold sensor chip as recognition species. A successful interaction of suspect hormone mimic with estrogen receptor is evidenced by net rise in SPR angle in real time. The assay has been validated in terms of optimum experimental conditions and specificity with estrogen as standard showing maximum estrogenic activity. As a proof of concept, proposed affinity assay is tested for screening the estrogenic activity of progesterone, pregnenolone, tamoxifen, and bisphenol-A as representative examples of potential EDCs of different classes.</p>
 
Publication, Distribution, Etc. Indian Journal of Chemistry -Section A (IJCA)
2020-10-06 10:36:53
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJCA/article/view/26726
 
Data Source Entry Indian Journal of Chemistry -Section A (IJCA); ##issue.vol## 59, ##issue.no## 5 (2020): INDIAN JOURNAL OF CHEMISTRY- SECTION A
 
Language Note en
 
Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJCA/article/download/26726/465482736
http://op.niscair.res.in/index.php/IJCA/article/download/26726/465482738
http://op.niscair.res.in/index.php/IJCA/article/download/26726/465482740
http://op.niscair.res.in/index.php/IJCA/article/download/26726/465482741
http://op.niscair.res.in/index.php/IJCA/article/download/26726/465482742
http://op.niscair.res.in/index.php/IJCA/article/download/26726/465482743
http://op.niscair.res.in/index.php/IJCA/article/download/26726/465482747
 
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