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<p><strong><em>In-Vitro </em></strong><strong>Incompatibility Study of Valsartan and Hydrochlorothiazide by Spectroscopic and RP-HPLC Method</strong></p>

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Title Statement <p><strong><em>In-Vitro </em></strong><strong>Incompatibility Study of Valsartan and Hydrochlorothiazide by Spectroscopic and RP-HPLC Method</strong></p>
 
Added Entry - Uncontrolled Name SEN, SANDIP ; SRIKRUPA INSTIITUTE OF PHARMACEUTICAL SCIENCES, SIDDIPET, TELANGANA-502277
SRIKRUPA INSTITUTE OF PHARMACEUTICAL SCIENCES
 
Uncontrolled Index Term Spectroscopy and Analytical Chemistry
Valsartan, Hydrochlorothiazide, Spectroscopic method, RP-HPLC, In-vitro drug interaction.
 
Summary, etc. The present investigation was based on an <em>in-vitro </em>incompatibility study between valsartan and hydrochlorothiazide by spectroscopic and reverse-phase high-pressure liquid chromatography (RP-HPLC) methods. The method was developed and validated by UltraViolet-Spectroscopic (UV-Spectroscopic) method using water and methanol (4:1) as the solvent. The RP-HPLC study was carried out using Acetonitrile, methanol, and 50Mm phosphate buffer (20:50:30%) adjusted to pH-3 with orthophosphoric acid. The methods showed linearity at the concentration range of 1-5μg/ml with correlation coefficient 0.9994 for valsartan and 0.9978 for hydrochlorothiazide in the method and 5-25 μg/ml in. Both the methods were shown a percentage relative standard deviation (%RSD) values less than 2. The <em>in-vitro </em>incompatibility study was carried out by  UV-Spectroscopic and RP-HPLC methods. The result of the study shown that there is a change in the concentration level of both drugs in combination. The structure of the interacting compound was determined by spectral analysis using IR, <sup>1</sup>HNMR and LC-MS study. The spectral analysis confirmed the formation of the new complex between valsartan and hydrochlorothiazide. The result obtained from the LC-MS study also correlates the reason behind an increase in the concentration of valsartan and decreases the concentration of hydrochlorothiazide during recovery study by spectroscopic and RP-HPLC method.
 
Publication, Distribution, Etc. Indian Journal of Chemistry -Section A (IJCA)
2020-10-06 11:31:09
 
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http://op.niscair.res.in/index.php/IJCA/article/view/31832
 
Data Source Entry Indian Journal of Chemistry -Section A (IJCA); ##issue.vol## 59, ##issue.no## 8 (2020): INDIAN JOURNAL OF CHEMISTRY- SECTION A
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJCA/article/download/31832/465497980
http://op.niscair.res.in/index.php/IJCA/article/download/31832/465498138
http://op.niscair.res.in/index.php/IJCA/article/download/31832/465498139
http://op.niscair.res.in/index.php/IJCA/article/download/31832/465500129
 
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