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Jasmonic acid (JA) induced-calcium elevation in Arabidopsis is highly variable due to time of day and conversion to JA-Ile

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Title Jasmonic acid (JA) induced-calcium elevation in Arabidopsis is highly variable due to time of day and conversion to JA-Ile
 
Creator Prajapati, Ramgopal
Mittal, Deepika
Meena, Mukesh Kumar
Vadassery, Jyothilakshmi
 
Subject Jasmonic acid
Calcium
Aequorin
Circadian
Arabidopsis
 
Description Accepted date: 23 September 2020
Plants have evolved mechanisms to effectively anticipate environmental changes via diurnal rhythmicity (day/night)
maintained by the circadian clock. Jasmonic acid biosynthesis and signalling are known to be under the control of the
circadian clock. Both JA and its bioactive form jasmonoyl-L-isoleucine (JA-Ile) when externally added can induce a
cytosolic Ca2? influx in Arabidopsis thaliana. JA and JA-Ile induced Ca2? is poorly understood and often used interchangeably to study Ca2? regulation of jasmonates. We attempted to understand if they are similar and if diurnal rhythms
or time of day regulate them. JA induced Cacyt
2? signature is variable according to time-of-day in Arabidopsis. JA is sensed
in two ways according to the time-of-day (a) directly sensed as JA and induces Cacyt
2? elevation (b) JA gets converted into
the JA-Ile by JAR1 and is sensed as JA-Ile, which we proved using jar1-1*aequorin. This twin sensing mode is responsible
for variability in JA induced Cacyt
2? signature. We further suggest caution when using JA as a stimulant for Cacyt
2? elevation
measurements to compare wild-type (Col-0 transformed with pMAQ2; transgenic aequorin) and effect of different
mutations. On the other hand bioactive JA-Ile induced Cacyt
2? signature is constant diurnally with maximum amplitude at
dawn which coincides with maximum sensitivity of JA-Ile receptor, COI1 and increased VSP2 expression. From the above
study we conclude that JA-Ile induced Cacyt
2? elevation is a better read-out than the highly variable JA-induced Cacyt
2?
elevations to study the output pathways.
We acknowledge Prof. Wilhelm Boland, Dr.
Guillermo Hugo Jimenez-Aleman and Dr. Axel Mitho¨fer for chemically synthesized JA-Ile, Dr. Michael Reichelt for phytohormone analysis, DBT-eLibrary Consortium (DeLCON) for providing access
to e-resources. This work was funded by MPG-India Partner Group in
Chemical Ecology of the Max Planck Society (Germany) and
Department of Science and Technology (DST), India for JV.
 
Date 2020-10-05T06:43:20Z
2020-10-05T06:43:20Z
2020
 
Type Article
 
Identifier Journal of Plant Biochemistry and Biotechnology, (In Press)
0971-7811
http://223.31.159.10:8080/jspui/handle/123456789/1107
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG