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Efficacy of Various Methods of Protein Extraction from Purslane Suitable for Proteome Analysis.

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Title Efficacy of Various Methods of Protein Extraction from Purslane Suitable for Proteome Analysis.
 
Creator Niharika, Shanker
Neha, S. Berde
Govindaraju, K.
Sukumar, Debnath
 
Subject 03 Proteins
23 Vegetables
 
Description The purslane leaf is characterized by high content of protein, vitamins and minerals. However, its use
is limited due to the high fiber content and anti-nutritional substances. In this perspective, the objective of this research
was to optimize the protein extraction processes, aiming the use of purslane leaves as an alternative protein. The
important characteristics of the protocol include, 1) coagulation of proteins with mild acid precipitation, 2) extraction
of protein by three-phase partitioning, 3) extraction of protein by TCA/Acetone method. The protein concentrations
among three methods were 802±0.2 µg/g in mild acid precipitation, 625±0.3 µg/g in TCA Acetone and 81.6±0.2 µg/g in
TPP, respectively. The solubilization of proteins (method 1) showed a higher extraction yield. Based on the literature
survey, the comparison of protocol for protein extraction from purslane leaf was demonstrated first time in this work
and showed reproducible results.
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14527/1/ijrar_issue_20542582.pdf
Niharika, Shanker and Neha, S. Berde and Govindaraju, K. and Sukumar, Debnath (2018) Efficacy of Various Methods of Protein Extraction from Purslane Suitable for Proteome Analysis. International Journal of Research and Analytical Reviews, 5 (4). pp. 451-456.