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Estimation of primary productivity (modified light and dark bottle oxygen method)

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Relation http://eprints.cmfri.org.in/4041/
 
Title Estimation of primary productivity (modified light and dark bottle oxygen method)
 
Creator Selvaraj, G S D
 
Subject Primary Production
Mangroves
 
Description This method is based on the estimation of
dissolved oxygen in the water samples (ml/l) by
Winkler’s method. Three BOD bottles are used for
this purpose. The DO content in the initial (I), Dark
(D) and Light (L) bottles with a capacity of 125 or
250 ml after specific incubation period are utilized to
calculate the primary productivity. The incubation
period various with the nature of water sample such
as 3.0 hours for sea water whereas 2.5 hours for
shallow estuarine water. The incubation is done in
light for the same in ‘L’ bottle and in dark ‘D’ bottle,
while sample in I bottle is fixed by winkler ‘A’ and
‘B’ at the initial stage of experiment. Gross Primary
(Photosynthetic) Production (GPP) is determined by
using L-D values extrapolated for 12 light hours of
the day. Net Primary (Photosynthetic) Production
(NPP) of oxygen is obtained from either L-I or 0.8
(L-D) value ( calculated for 12 light hours of the day)
whichever comes closer to (but less than) L-D value.
If the L-I value falls much below 0.8 (L-D) value, it
indicates that bacterial interference ( other
biochemical oxidation process ) is more in the water
samples. At times, L-I value exceeds L-D value. In
such cases , 0.8 (L-D) value should be taken into
account to assess NPP.
 
Publisher CMFRI; Kochi
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/4041/1/16.pdf
Selvaraj, G S D (2005) Estimation of primary productivity (modified light and dark bottle oxygen method). CMFRI Special Publication Mangrove ecosystems: A manual for the assessment of biodiversity, 83. pp. 199-200.