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<p><strong>A novel FRET probe for determination of fluorescein sodium in aqueous solution: Analytical application for ophthalmic sample</strong></p>

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Title Statement <p><strong>A novel FRET probe for determination of fluorescein sodium in aqueous solution: Analytical application for ophthalmic sample</strong></p>
 
Added Entry - Uncontrolled Name Patil, Dhanshri ; Assistant Professor, Department of Chemistry, Krishna Mahavidyalaya, Rethare Bk-415108
Patil, Vishal ; Assistant Professor, Sanjeevan Engineering and TechnologyInstitute, Panhala 416 201
 
Uncontrolled Index Term Analytical Chemistry
Fluorescent pyrene nanoparticles, fluorescein sodium, fluorescence resonance energy transfer
 
Summary, etc. <p>Fluorescent pyrene nanoparticles (PyNPs) were prepared by a reprecipitation method in the presence of sodium dodecyl sulphate (SDS) as a stabilizer. The formation of PyNPs was confirmed by dynamic light scattering (DLS), UV-visible absorption spectroscopy, fluorescence spectroscopy and excited state lifetime measurements. DLS results of PyNPs shows a narrow size distribution with average particle size of 77.4 nm and negative zeta potential. The systematic FRET experiments performed by measuring fluorescence quenching of PyNPs with successive addition of FL-Na analyte exploited the use of PyNPs as nanoprobe for detection of FL-Na in aqueous solution. The fluorescence of PyNPs was quenched by Fl-Na and quenching is in accordance with the Stern-Volmer relation. The distance r between the donor (PyNPs) and acceptor (FL-Na) molecules was obtained according to the fluorescence resonance energy transfer. The fluorescence quenching results were used further to develop an analytical method for estimation of fluorescein sodium from ophthalmic samples available commercially in the market.</p>
 
Publication, Distribution, Etc. Indian Journal of Chemistry -Section A (IJCA)
2020-10-12 16:17:58
 
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http://op.niscair.res.in/index.php/IJCA/article/view/24643
 
Data Source Entry Indian Journal of Chemistry -Section A (IJCA); ##issue.vol## 58, ##issue.no## 11 (2019): INDIAN JOURNAL OF CHEMISTRY- SECTION A
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJCA/article/download/24643/465476970
http://op.niscair.res.in/index.php/IJCA/article/download/24643/465476971
 
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