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Sequestering miR165/166 enhances seed germination in Arabidopsis thaliana under normal condition and ABA treatment

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Title Sequestering miR165/166 enhances seed germination in Arabidopsis thaliana under normal condition and ABA treatment
 
Creator Sarkar Das, Shabari
Majee, Manoj
Nandi, Asis K.
Karmakar, Prakash
 
Subject Seed germination
miR165/166
Small RNA
miRNA
Stress response
ABA
Arabidopsis
 
Description Accepted dated: 9 September 2020
Seed germination is a dynamic process involving imbibition, increased metabolic activity and protrusion of a tiny plantlet
rupturing the seed coat. Many genes, phytohormones like ABA and GA have been implicated in germination of Arabidopsis thaliana seeds. Although many microRNAs (miRNAs) have been shown to be differentially expressed during
seed germination process, their role remains mostly unaddressed. Here we address the role of developmentally important
miR165/166 in the process of seed germination. We demonstrate that the seeds of transgenic A. thaliana having target
mimic-miR165/166 (eTM-miR165/166), where miR165/166 is sponged, show better germination efficiency. The seeds of
this line also maintain better germination even under ABA treatment, which is a negative regulator of seed germination.
Thus, our results suggest that, sequestering miR165/166 activity enhances seed germination efficiency under normal and
ABA-stress condition.
SSD and MM acknowledge financial and
infrastructural support from NIPGR. SSD acknowledges DSTWomen Scientist-A (WOS-A) fellowship from the Department of
Science and Technology (DST), India (WOS-A/LS-1276/2014). We
thank Dr A. K. Sarkar (NIPGR) for plant materials and support. PK,
AKN, SSD thank Vidyasagar University for support.
 
Date 2020-10-15T09:39:55Z
2020-10-15T09:39:55Z
2020
 
Type Article
 
Identifier Journal of Plant Biochemistry and Biotechnology, (In Press)
0971-7811
https://doi.org/10.1007/s13562-020-00607-3
https://link.springer.com/article/10.1007/s13562-020-00607-3
http://223.31.159.10:8080/jspui/handle/123456789/1115
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG