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MKK3-MPK6-MYC2 module positively regulates ABA biosynthesis and signalling in Arabidopsis

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Title MKK3-MPK6-MYC2 module positively regulates ABA biosynthesis and signalling in Arabidopsis
 
Creator Verma, Deepanjali
Bhagat, Prakash Kumar
Sinha, Alok Krishna
 
Subject ABA
MYC2
ABA2
MPK6
MKK3
Seed germination
 
Description Accepted date: 6 October 2020
Involvement of MAPK cascades is well studied in the regulation of ABA mediated responses such as stomatal opening,
seed germination and stress tolerance. However most of the studies have focused on ABA signalling and involvement of
MAPKs in ABA biosynthesis remains elusive. Hence, in the current study, we have identified the new role of MKK3-
MPK6-MYC2 module in positive regulation of ABA biosynthesis as well as ABA responsive genes. Seed germination
studies disclosed the ABA insensitive phenotype of myc2, mpk6 and mkk3 mutants. Reduced expression of ABA
biosynthesis genes, ABA2 and NCED3 in myc2 and MAPK cascade mutants was confirmed by witnessing the direct binding
of MYC2 to the promoter of ABA2 in EMSA assays. Additionally, ABA responsive genes also displayed altered expression
patterns in these mutants. Further, transcript and biochemical studies validated the role of MPK6 upstream to MYC2 and
downstream to MKK3 in response to ABA. Altogether, we have shown that MKK3-MPK6-MYC2 module induces the
expression of several ABA related genes and regulate ABA mediated inhibition of seed germination.
This work was supported from the core grant of
National Institute of Plant Genome Research, New Delhi from
Department of Biotechnology, Government of India. DV is thankful
to University Grant Commission and PKB thanks Department of
Biotechnology, Government of India for fellowships. Authors thank
Prof. Sudip Chattopadhyay for sharing Atmyc2 mutant MYC2OE
seeds. The work was supported by National Institute of Plant
Genome Research (NIPGR).
 
Date 2020-10-23T10:17:40Z
2020-10-23T10:17:40Z
2020
 
Type Article
 
Identifier Journal of Plant Biochemistry and Biotechnology, (In Press)
0971-7811
https://doi.org/10.1007/s13562-020-00621-5
https://link.springer.com/article/10.1007/s13562-020-00621-5
http://223.31.159.10:8080/jspui/handle/123456789/1118
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG