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RS_CRZ1, a C2H2 type transcription factor is required for pathogenesis of Rhizoctonia solani AG1-IA in tomato

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Title RS_CRZ1, a C2H2 type transcription factor is required for pathogenesis of Rhizoctonia solani AG1-IA in tomato
 
Creator Ghosh, Srayan
Kant, Ravi
Pradhan, Amrita
Jha, Gopaljee
 
Subject sheath blight disease
pathogenicity determinant
infection cushion
metal ion stress
VIGS
 
Description Accepted date: 8 October 2020
Rhizoctonia solani is a necrotrophic fungal pathogen which causes disease in diverse plant species. In recent years, the genomic and transcriptomic studies have identified several candidate pathogenicity determinants of R. solani; however, most of them remain to be validated. In this study, we report a viral vector-based host induced gene silencing (HIGS) as well as a dsRNA (double stranded RNA) based approach to effectively downregulate genes of R. solani AG1-IA (BRS1 strain) during pathogenesis in tomato. We tested a few of the in-planta upregulated R. solani genes and observed that silencing of one of them i.e. RS_CRZ1 (a C2H2 type Zn finger transcription factor) significantly compromises the pathogenesis of R. solani in tomato. The RS_CRZ1 silenced plants not only exhibited significant reduction in disease symptoms, but the depth of pathogen colonization was also compromised. Furthermore, we identified the R. solani genes that were co-regulated with RS_CRZ1 during pathogenicity process. The HIGS mediated silencing of a few of them (CL1756Contig1; subtilisin like protease and CL1817Contig2; 2OG- Fe(II) oxygenase) compromised the pathogenesis of R. solani in tomato. The ectopic expression of RS_CRZ1 complemented the crz1 mutant of yeast and restored tolerance against various metal ion stress. Overall, our study reveals the importance of RS_CRZ1 in managing the hostile environment encountered during host colonization. Also, it emphasizes the relevance of HIGS and dsRNA-based gene silencing approach towards functional characterization of pathogenicity determinants of R. solani.
SG was supported by SPM fellowship from Council of Scientific and Industrial
Research (Govt. of India), RK was supported by N-PDF fellowship from SERB, (DST,
Govt of India) and AP was supported by CSIR-UGC fellowship from University Grants
Commission (Govt. of India). We thank Dr. Senthil-Kumar Muthappa, NIPGR, New
Delhi for providing the pTRV (pTRV1+pTRV2) strains. The authors are also thankful
to DBT-eLibrary Consortium (DeLCON) for providing access to e-resources. The
assistance of central instrumentation facilities for sequencing, qRT-PCR, and
microscopic (confocal and stereo-zoom) analysis are acknowledged. This work was
supported by core research grant from the National Institute of Plant Genome
Research, India and research funding from DBT, Government of India. The authors
also acknowledge DBT sponsored NIPGR flagship program “Imparting sheath blight
tolerance in rice” (SAN No.102/IFD/SAN/763/2019-20) to support research in GJ lab.
The funders had no role in study design, data collection and analysis, decision to
publish, or preparation of the manuscript. The authors declare no conflict of interest.
 
Date 2020-10-23T09:42:13Z
2020-10-23T09:42:13Z
2021
 
Type Article
 
Identifier Molecular Plant-Microbe Interactions, (In Press)
1943-7706
https://doi.org/10.1094/MPMI-05-20-0121-R
https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-20-0121-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed
http://223.31.159.10:8080/jspui/handle/123456789/1117
 
Language en_US
 
Format application/pdf
 
Publisher American Phytopathological Society