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<p>Graphene oxide-supported gold nanocomposites for highly sensitive sandwich immunosensor for α-fetoprotein detection</p>

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Title Statement <p>Graphene oxide-supported gold nanocomposites for highly sensitive sandwich immunosensor for α-fetoprotein detection</p>
 
Added Entry - Uncontrolled Name Li, Pengjun ; Department of Chemistry, Nanchang University, Nanchang 330031, China
Lai, Juanhua ; Department of Chemistry, Nanchang University, Nanchang 330031, China
Qiu, Ping ; Department of Chemistry, Nanchang University, Nanchang 330031, China
the Open Funds of the State Key Laboratory of Electroanalytical Chemistry(SKLEAC201602)
 
Uncontrolled Index Term analytical chemistry
Nanocomposites, Immunosensors, Gold nanoparticles, Sandwich immunosensors, Graphene oxide, α-Fetoprotein
 
Summary, etc. <p style="text-align: justify;">A graphene oxide-Au NPs sensor platform combined with a multiple-enzyme labeled detection antibody-carbon sphere bioconjugate has been used as the basis for an ultrasensitive electrochemical immunosensor to detect the cancer biomarker, α-fetoprotein (AFP). Greatly enhanced sensitivity has been achieved by using the bioconjugates featuring horseradish peroxidase (HRP) and Ab2 linked to carbon nanospheres (Ab2-HRP-Au-PDA-carbon sphere) at a high ratio of HRP/Ab2. After a sandwich immunoreaction, the Ab2-HRP-Au-PDA-carbon sphere captured onto the electrode surface produces an amplified electrocatalytic response due to the reduction of enzymatically oxidized thionine in the presence of hydrogen peroxide. The increase of response current is proportional to the AFP concentration in the range of 0.01-20 ng/mL,<sup> </sup>with <br /> the detection limit of 3 pg/mL. This amplification strategy is a promising platform for detection of other proteins and <br /> clinical applications.</p>
 
Publication, Distribution, Etc. Indian Journal of Chemistry -Section A (IJCA)
2020-10-29 11:16:02
 
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http://op.niscair.res.in/index.php/IJCA/article/view/14955
 
Data Source Entry Indian Journal of Chemistry -Section A (IJCA); ##issue.vol## 56, ##issue.no## 4 (2017): INDIAN JOURNAL OF CHEMISTRY - SECTION A
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJCA/article/download/14955/46006
http://op.niscair.res.in/index.php/IJCA/article/download/14955/46007
 
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