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Improved performance of immobilized lipase from optimized biosupport material (polyvinyl alcohol/AlgNa) and its characterization

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Title Improved performance of immobilized lipase from optimized biosupport material (polyvinyl alcohol/AlgNa) and its characterization
 
Creator Kumar, Dilip
Giri, Balendu Shekher
Verma, Bhawna
 
Subject Biosupport catalyst
Entrapment
Loading efficiency
Pseudomonas cepacia
Transesterification
 
Description 803-810
Enzymes play an imminent role as biocatalyst for various biotechnological applications as well as production of biodiesel. Here, we focused on the preparation of biosupport materials (polyvinyl alcohol/AlgNa) and their use in immobilization of lipase from Pseudomonas cepacia. Lipase was successfully immobilized onto polyvinyl alcohol/AlgNa in the form of biosupport materials by entrapment method. The mechanical strength, swelling ratio, thermal properties, optimum temperature and pH, lipase loading, leaching, immobilization yields and activity, characterization of the support materials were performed. The optimized pH and temperature for free lipase were 8.0 and 40°C, respectively, while the best pH and temperature for polyvinyl alcohol/AlgNa immobilized lipase were 8.0 and 50°C. 73.12% of the initial activity was retained for the immobilized biosupport catalyst in 6 cycles. The biosupport catalyst beads showed a fascinating degree of immobilized lipase activity along with high immobilization yield. The highest immobilized lipase activity and loading efficiency found to be 87.28 (U/g) and 55.2%, respectively. SEM analysis confirms the development of macro-porous structure from the combination of alginate. No sharp chemical interaction was observed in the behaviour of the functional groups of polyvinyl alcohol and AlgNa in the polyvinyl alcohol/sodium alginate blends, which were confirmed from Fourier transform infrared (FTIR) spectra. The immobilized biosupport catalysts are easily separable, recyclable and could be frequently used for transesterification.
 
Date 2020-10-29T10:45:57Z
2020-10-29T10:45:57Z
2020-11
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/55578
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJEB Vol.58(11) [November 2020]