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Role of polyamines on in vitro regeneration and podophyllotoxin production in Podophyllum hexandrum Royle

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Title Role of polyamines on in vitro regeneration and podophyllotoxin production in Podophyllum hexandrum Royle
 
Creator Rajesh, Manoharan
Sivanandhan, Ganeshan
Kapildev, Gnanajothi
Ganapathi, Andy
 
Subject Indian Mayapple
Mass culture
PTOX
Putrescine
Shoot production
Spermidine
Zygotic embryo
 
Description 777-787
Podophyllum hexandrum is a popular medicinal plant endemic to Himalayas. In the present investigation, we report an efficient mass propagation protocol and Podophyllotoxin (PTOX) production in in vitro-derived plants of P. hexandrum, cultured on MS medium containing various plant growth regulators and polyamines. A combination of BA (1.5 mg/L), IAA (0.2 mg/L) and spermidine (20 mg/L) produced the highest number of multiple shoots per explant (23 shoots) after 6 weeks of culture; the regenerated shoots were elongated on the same medium. The elongated-shoots were rooted on root induction medium (half strength MS medium) supplemented with IBA (1.5 mg/L) and putrescine (15 mg/L). The rooted plantlets were successfully hardened and acclimatized with a survival rate of 68% in the greenhouse. The highest content of PTOX (4.23 mg/g DW) was recorded in in vitro derived roots followed by leaves when compared to field-grown parent plants. The present system offers the possibility to use in vitro culture techniques for mass propagation and PTOX production for commercial utilization.
 
Date 2020-10-29T10:53:42Z
2020-10-29T10:53:42Z
2020-11
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/55581
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJEB Vol.58(11) [November 2020]