Record Details

<p><em>Rhizobium rhizogenes</em> Mediated Hairy Root Transformation and Analysis of Secondary Metabolites in <em>Phlogacanthus thyrsiflorus </em>Nees Hairy Roots using GC-MS</p>

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement <p><em>Rhizobium rhizogenes</em> Mediated Hairy Root Transformation and Analysis of Secondary Metabolites in <em>Phlogacanthus thyrsiflorus </em>Nees Hairy Roots using GC-MS</p>
 
Added Entry - Uncontrolled Name Singh, Shweta ; Department of Bio-Engineering, Birla institute of Technology, Mesra, Ranchi 835 215, Jharkhand, India
Banerjee, Madhuparna ; College of Biotechnology, Birsa Agricultural University, Kanke, Ranchi 834 006, Jharkhand, India
Kumar, Manish ; Department of Bio-Engineering, Birla institute of Technology, Mesra, Ranchi 835 215, Jharkhand, India
 
Uncontrolled Index Term rol b; rol c; GC-MS; Strain A4
 
Summary, etc. <p class="Abstract">An efficient transformation system was developed for <em>Phlogacanthus thyrsiflorus, </em>a medicinal plant of northeast India, using <em>Rhizobium rhizogenes</em> strains A4 for infecting shoot tip, nodes and leaf explants derived from aseptically raised plants. The transformation frequency was found dependent on the explants as well as the co-culture periods. Highest transformation frequency of 95.69% was obtained from shoot tip explants co-cultured for 4 hours. An average of 6.37 hairy roots per explant were obtained from shoot tip explants within three weeks of infection. Whereas, transformation frequency along with number of transformed roots were fewer in node and leaf explants. The presence of T<sub>L</sub> DNA of <em>R. rhizogenes</em> strains A4 in the hairy root genome of <em>P. thyrsiflorus </em>was confirmed through PCR based cloning and sequencing in which the sequencing results of the PCR amplicon confirmed the presence of <em>rol b</em> and <em>rol c</em> genes in the host genome.<em> </em>Sequence results for <em>rol </em>genes were found to be 97 percent and 99 percent similar with <em>rol b</em> and <em>rol c</em> gene present in NCBI database. GC-MS analysis of methanolic extract of the hairy roots reveals the presence of a few medicinally important secondary metabolites.</p>
 
Publication, Distribution, Etc. Journal of Scientific and Industrial Research (JSIR)
2020-11-09 16:10:07
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/JSIR/article/view/40467
 
Data Source Entry Journal of Scientific and Industrial Research (JSIR); ##issue.vol## 79, ##issue.no## 7
 
Language Note en