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Optimizing Medium Components to Enhance High Cell Mass Production of Biotherapeutic Strai <em>Lactobacillus reuteri</em> DSM 20016T by Statistical Method

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Title Statement Optimizing Medium Components to Enhance High Cell Mass Production of Biotherapeutic Strai <em>Lactobacillus reuteri</em> DSM 20016T by Statistical Method
 
Added Entry - Uncontrolled Name Selvamani, S ; Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81 310, Skudai, Johor, Malaysia
Dailin, D J; Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81 310, Skudai, Johor, Malaysia
Rostom, M ; Academy of Scientific Research and Technology, ASRT, Cairo, Egypt
Malek, R A; Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81 310, Skudai, Johor, Malaysia
Gupta, V K; Department of Chemistry and Biotechnology, ERA Chair of Green Chemistry, Tallinn University of Technology, Tallinn, Estonia
El-Enshasy, H A; City of Scientific Research and Technology Applications (CSAT), New Burg Al Arab Alexandria, Egypt
 
Uncontrolled Index Term Probiotic cultivation; High cell mass production; Medium optimization
 
Summary, etc. <p style="text-align: justify;">Probiotics referred to a group of living microorganisms which highly influence the human health. A number of studies have highlighted on the bio-therapeutic potential of <em>Lactobacillus reuteri</em> strains, especially in treating eradication of <em>Helicobacter pylori</em>. Therefore, in present investigation, statistical methods were applied to optimize medium composition for high cell mass production of <em>L. reuteri</em> strain DSM 20016<sup>T</sup>. Most influencing medium components were screened by using Plackett-Burman and optimized using Box-Bhenken experimental design. The concentration of lactose, yeast extract and phosphate in cultivation medium has shown significant effect on the cell mass production. The highest cell mass obtained after 48h incubation was 3.96 ± 0.02 gL<sup>−1</sup> in RSM-optimized medium compared to 1.76 ± 0.17 gL<sup>−1</sup> in un-optimized medium.</p>
 
Publication, Distribution, Etc. Journal of Scientific and Industrial Research (JSIR)
2020-11-09 17:14:07
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/JSIR/article/view/41715
 
Data Source Entry Journal of Scientific and Industrial Research (JSIR); ##issue.vol## 79, ##issue.no## 9 (20)
 
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