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Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet

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Relation http://oar.icrisat.org/11638/
https://doi.org/10.1038/s41598-020-76230-y
doi:10.1038/s41598-020-76230-y
 
Title Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet
 
Creator Pujar, M
Gangaprasad, S
Govindaraj, M
Gangurde, S S
Kanatti, A
Kudapa, H
 
Subject Pearl Millet
Food and Nutrition
 
Description Pearl millet hybrids biofortified with iron (Fe) and zinc (Zn) promise to be part of a long-term strategy
to combat micronutrient malnutrition in the arid and semi-arid tropical (SAT) regions of the world.
Biofortification through molecular breeding is the way forward to achieving a rapid trait-based
breeding strategy. This genome-wide association study (GWAS) was conducted to identify significant
marker-trait associations (MTAs) for Fe, Zn, and protein content (PC) for enhanced biofortification
breeding. A diverse panel of 281 advanced inbred lines was evaluated for Fe, Zn, and PC over two
seasons. Phenotypic evaluation revealed high variability (Fe: 32–120 mg kg−1, Zn: 19–87 mg kg−1, PC:
8–16%), heritability (hbs2 ≥ 90%) and significantly positive correlation among Fe, Zn and PC (P = 0.01),
implying concurrent improvement. Based on the Diversity Arrays Technology (DArT) seq assay, 58,719
highly informative SNPs were filtered for association mapping. Population structure analysis showed
six major genetic groups (K = 6). A total of 78 MTAs were identified, of which 18 were associated with
Fe, 43 with Zn, and 17 with PC. Four SNPs viz., Pgl04_64673688, Pgl05_135500493, Pgl05_144482656,
and Pgl07_101483782 located on chromosomes Pgl04 (1), Pgl05 (2) and Pgl07 (1), respectively were
co-segregated for Fe and Zn. Promising genes, ‘Late embryogenesis abundant protein’, ‘Myb domain’,
‘pentatricopeptide repeat’, and ‘iron ion binding’ coded by 8 SNPs were identified. The SNPs/genes
identified in the present study presents prospects for genomics assisted biofortification breeding in
pearl millet.
 
Publisher Nature Research
 
Date 2020-11
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11638/1/GWAS%20Scientific%20reports%20s41598-020-76230-y.pdf
Pujar, M and Gangaprasad, S and Govindaraj, M and Gangurde, S S and Kanatti, A and Kudapa, H (2020) Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet. Scientific Reports (TSI), 10 (1). ISSN 2045-2322