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Development of a multiplex PCR for the identification of Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Streptococcus agalactiae in milk

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Title Development of a multiplex PCR for the identification of Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Streptococcus agalactiae in milk
 
Creator Sekhri, Isha
Chandra, Mudit
Kaur, Gurpreet
Narang, Deepti
Arora, A. K.
 
Subject Mastitis milk
Microorganisms
Multiplex PCR
 
Description 125-130
In the dairy industry financial losses mainly due to loss of milk production caused by mastitis and mastitis related problems is very common. In the mastitis, identification of the infectious agent/s could be of great help. In the present study, a multiplex polymerase chain reaction (PCR) to identify five common organisms implicated in the mastitis was tried. Primers for identification of Staphylococcus aureus, Escherichia coli (E. coli), Klebsiella pneumoniae, Pseudomonas aeruginosa, and Streptococcus agalactiae were selected/ designed. DNA was extracted from the affected milk using two different methods and was subjected to multiplex PCR. It was observed from the study that a multiplex PCR to amplify four organisms E. coli, K. pneumoniae, S. agalactiae and P. aeruginosa was developed successfully to quicken the detection of common mastitis causing organisms.
 
Date 2020-11-13T06:36:08Z
2020-11-13T06:36:08Z
2020-04
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/55651
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBT Vol.19(2) [April 2020]