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Constitutive expression of protease inhibitor gene isolated from black gram (Vigna mungo L.) confers resistance to Spodoptera litura in transgenic tobacco plants

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Title Constitutive expression of protease inhibitor gene isolated from black gram (Vigna mungo L.) confers resistance to Spodoptera litura in transgenic tobacco plants
 
Creator Jagdish, Mudiyappanayar
Koundal, K R
 
Subject Insect resistance
Vigna mungo
Protease inhibitor
Agrobacterium tumefaciens
Transgenic tobacco
 
Description 94-101
Plant insecticidal proteins like protease inhibitors have been reported to confer insect resistance in transgenic plants. We have cloned a protease inhibitor gene of black gram (Vigna mungo L.) and developed transgenic tobacco plants expressing the protease inhibitor constitutively under the 35S promoter by Agrobacterium tumefaciens mediated genetic transformation. A full-length open reading frame (ORF) of 545 nucleotides codes for a serine protease inhibitor protein with a deduced sequence of 180 amino acids contributing to a predicted molecular mass of 20.2 kDa. The Vigna mungo protease inhibitor (VmPI) is basically a Bowman-Birk type proteinase inhibitor (BBI) of double headed type; family of plant serine protease inhibitors that block trypsin or chymotrypsin. Comparison for conserved domain in the protein by National Centre for Biotechnology Information (NCBI) conserved domain (CD) search showed that the regions from 113-168 amino acid residues have the conserved domain of which 9 residues compose reactive loops on the conserved domain of BBI. The transgenic tobacco plants developed were confirmed by Southern, northern hybridization, polymerase chain reaction (PCR) and reverse transcriptase-PCR (RT-RCR) analysis. When transgenic T1 tobacco plants were analyzed for insecticidal bioassays, the larval growth of Spodoptera litura, the tobacco cutworm was found severely retarded as compared to their growth on control plants. Therefore, black gram protease inhibitor could be used as an alternative individually or in combination with other insecticidal genes for the development of insect-resistant transgenic crop plants.
 
Date 2020-11-13T07:05:17Z
2020-11-13T07:05:17Z
2020-04
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/55655
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBT Vol.19(2) [April 2020]