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Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence

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Relation http://oar.icrisat.org/11643/
https://doi.org/10.3390/genes11091026
doi:10.3390/genes11091026
 
Title Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence
 
Creator Kiranmayee, K N S U
Hash, C T
Sivasubramani, S
Ramu, P
Amindala, B P
Rathore, A
Kishor, P B K
Gupta, R
Deshpande, S P
 
Subject Sorghum
Genetics and Genomics
 
Description This study was conducted to dissect the genetic basis and to explore the candidate genes
underlying one of the important genomic regions on an SBI-10 long arm (L), governing the complex
stay-green trait contributing to post-flowering drought-tolerance in sorghum. A fine-mapping
population was developed from an introgression line cross—RSG04008-6 (stay-green) J2614-11
(moderately senescent). The fine-mapping population with 1894 F2 was genotyped with eight SSRs
and a set of 152 recombinants was identified, advanced to the F4 generation, field evaluated with three
replications over 2 seasons, and genotyped with the GBS approach. A high-resolution linkage map
was developed for SBI-10L using 260 genotyping by sequencing—Single Nucleotide Polymorphism
(GBS–SNPs). Using the best linear unpredicted means (BLUPs) of the percent green leaf area (%GL)
traits and the GBS-based SNPs, weidentified seven quantitative trait loci (QTL) clusters and single gene,
mostly involved in drought-tolerance, for each QTL cluster, viz., AP2/ERF transcription factor family
(Sobic.010G202700), NBS-LRR protein (Sobic.010G205600), ankyrin-repeat protein (Sobic.010G205800),
senescence-associated protein (Sobic.010G270300), WD40 (Sobic.010G205900), CPK1 adapter protein
(Sobic.010G264400), LEA2 protein (Sobic.010G259200) and an expressed protein (Sobic.010G201100).
The target genomic region was thus delimited from 15 Mb to 8 genes co-localized with QTL clusters,
and validated using quantitative real-time (qRT)–PCR.
 
Publisher MDPI
 
Date 2020-09
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11643/1/genes-11-01026-v2.pdf
Kiranmayee, K N S U and Hash, C T and Sivasubramani, S and Ramu, P and Amindala, B P and Rathore, A and Kishor, P B K and Gupta, R and Deshpande, S P (2020) Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence. Genes (TSI), 11 (1026). pp. 1-26. ISSN 2073-4425