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Discovery and validation of candidate genes for grain iron and zinc metabolism in pearl millet [Pennisetum glaucum (L.) R. Br.]

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Relation http://oar.icrisat.org/11647/
https://doi.org/10.1038/s41598-020-73241-7
doi:10.1038/s41598-020-73241-7
 
Title Discovery and validation of candidate genes for grain iron and zinc metabolism in pearl millet [Pennisetum glaucum (L.) R. Br.]
 
Creator Mahendrakar, M D
Parveda, M
Kishor, P B K
Srivastava, R K
 
Subject Pearl Millet
Genetics and Genomics
Food and Nutrition
 
Description Pearl millet is an important crop for alleviating micronutrient malnutrition through genomics-assisted
breeding for grain Fe (GFeC) and Zn (GZnC) content. In this study, we identified candidate genes
related to iron (Fe) and zinc (Zn) metabolism through gene expression analysis and correlated it with
known QTL regions for GFeC/GZnC. From a total of 114 Fe and Zn metabolism-related genes that were
selected from the related crop species, we studied 29 genes. Different developmental stages exhibited
tissue and stage-specific expressions for Fe and Zn metabolism genes in parents contrasting for GFeC
and GZnC. Results revealed that PglZIP, PglNRAMP and PglFER gene families were candidates for
GFeC and GZnC. Ferritin-like gene, PglFER1 may be the potential candidate gene for GFeC. Promoter
analysis revealed Fe and Zn deficiency, hormone, metal-responsive, and salt-regulated elements.
Genomic regions underlying GFeC and GZnC were validated by annotating major QTL regions for grain
Fe and Zn. Interestingly, PglZIP and PglNRAMP gene families were found common with a previously
reported linkage group 7 major QTL region for GFeC and GZnC. The study provides insights into the
foundation for functional dissection of different Fe and Zn metabolism genes homologs and their
subsequent use in pearl millet molecular breeding programs globally.
 
Publisher Nature Research
 
Date 2020-10
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11647/1/s41598-020-73241-7.pdf
Mahendrakar, M D and Parveda, M and Kishor, P B K and Srivastava, R K (2020) Discovery and validation of candidate genes for grain iron and zinc metabolism in pearl millet [Pennisetum glaucum (L.) R. Br.]. Scientific Reports (TSI), 10 (1). ISSN 2045-2322