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Conserved LBL1-ta-siRNA and miR165/166-RLD1/2 modules regulate root development in maize

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Title Conserved LBL1-ta-siRNA and miR165/166-RLD1/2 modules regulate root development in maize
 
Creator Gautam, Vibhav
Singh, Archita
Yadav, Sandeep
Singh, Sharmila
Kumar, Pramod
Das, Shabari Sarkar
Sarkar, Ananda K.
 
Subject small RNA
ta-siRNA
LBL1
root development
vascular patterning
maize
 
Description Accepted date: November 2, 2020
Root system architecture and anatomy of monocotyledonous maize is significantly different from
dicotyledonous model Arabidopsis. The molecular role of non-coding RNA (ncRNA) is poorly
understood in maize root development. Here we address the role of LEAFBLADELESS1
(LBL1), a component of maize trans-acting short-interfering RNA (ta-siRNA), in maize root
development. We report that the root growth, anatomical patterning, number of lateral roots
(LRs) and monocot-specific crown roots (CRs) and seminal roots (SRs) are significantly affected
in lbl1-rgd1 mutant, which is defective in production of ta-siRNA, including tasiR-ARF that
targets AUXIN RESPONSE FACTOR3 (ARF3) in maize. Altered accumulation and distribution
of auxin, due to differential expression of auxin biosynthesis and transporter genes, created an
imbalance in auxin signaling. Altered expression of microRNA165/166 (miR165/166) and its
targets ROLLED1/2 (RLD1/2) contributed to the changes in lbl1-rgd1 root growth and vascular
patterning, as was evident by altered root phenotype of Rld1-O semi-dominant mutant.
Thus, LBL1/ta-siRNA module regulates root development, possibly by affecting auxin
distribution and signaling, in crosstalk with miR165/166-RLD1/2 module. We further showed
that ZmLBL1 and its Arabidopsis homolog AtSGS3 proteins are functionally conserved.
VG, ArS, SS, PK thanks, Council of Scientific and Industrial Research (CSIR, Govt. of India),
New Delhi India for fellowship. SY thanks University Grants Commission (UGC, India) and
National Institute of Plant Genome Research, New Delhi (NIPGR) for fellowship. SSD
acknowledges Vidyasagar University for support. AKS acknowledges NIPGR for funding and
necessary infrastructural setups. We acknowledge the Central Instrument Facility (NIPGR, New
Delhi) for real time PCR, microtome, and light/confocal microscopes, plant growth facility, and
other facilities. We sincerely thank Prof. Marja Timmermans (CSHL, USA; currently, Univ. of
Tubingen, Germany) for plant material support and valuable critical comments on the
manuscript. We thank Prof. Klaus Palme for providing PIN1 antibody. We acknowledge the
DBT‐eLibrary Consortium (DeLCON) for providing access to e‐resources. We also
acknowledge ABRC, TAIR and Maize GDB for plant material/ genomic sequence resources.
 
Date 2020-11-12T05:48:48Z
2020-11-12T05:48:48Z
2020
 
Type Article
 
Identifier Development, (In Press)
1477-9129
https://doi.org/10.1242/dev.190033
https://dev.biologists.org/content/early/2020/11/08/dev.190033
http://223.31.159.10:8080/jspui/handle/123456789/1123
 
Language en_US
 
Format application/pdf
 
Publisher The Company of Biologists