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p-Coumaric acid inhibits the Listeria monocytogenes RecA protein functions and SOS response: An antimicrobial target.

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Relation http://ir.cftri.com/14613/
https://doi.org/10.1016/j.bbrc.2019.07.093
 
Title p-Coumaric acid inhibits the Listeria monocytogenes RecA protein
functions and SOS response: An antimicrobial target.
 
Creator Debika, Ojha
Neelakanteshwar Patil, K.
 
Subject 11 Antibiotics Chemistry
02 Bacteriology
 
Description Bacterial RecA plays an important role in the evaluation of antibiotic resistance via stress-induced DNA
repair mechanism; SOS response. Accordingly, RecA became an important therapeutic target against
antimicrobial resistance. Small molecule inhibitors of RecA may prevent adaptation of antibiotic resistance mutations and the emergence of antimicrobial resistance. In our study, we observed that phenolic
compound p-Coumaric acid as potent RecA inhibitor. It inhibited RecA driven biochemical activities
in vitro such as ssDNA binding, strand exchange, ATP hydrolysis and RecA coprotease activity of E. coli and
L. monocytogenes RecA proteins. The mechanism underlying such inhibitory action of p-Coumaric acid
involves its ability to interfere with the DNA binding domain of RecA protein. p-Coumaric acid also
potentiates the activity of ciprofloxacin by inhibiting drastic cell survival of L. monocytogenes as well as
filamentation process; the bacteria defensive mechanism in response to DNA damage. Additionally, it
also blocked the ciprofloxacin induced RecA expression leading to suppression of SOS response in
L. monocytogenes. These findings revealed that p-Coumaric acid is a potent RecA inhibitor, and can be
used as an adjuvant to the existing antibiotics which not only enhance the shelf-life but also slow down
the emergence of antibiotic resistance in bacteria.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14613/1/Biophysical%20Research%20Communications%20517%20%282019%29%20655e661.pdf
Debika, Ojha and Neelakanteshwar Patil, K. (2019) p-Coumaric acid inhibits the Listeria monocytogenes RecA protein functions and SOS response: An antimicrobial target. Biochemical and Biophysical Research Communications, 517. pp. 655-661.