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Enhanced phosphorylation of AMPK by lutein and oxidised lutein that lead to mitochondrial biogenesis in hyperglycemic HepG2 cells.

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Relation http://ir.cftri.com/14631/
https://doi.org/10.1002/jcb.28793
 
Title Enhanced phosphorylation of AMPK by lutein and oxidised
lutein that lead to mitochondrial biogenesis in
hyperglycemic HepG2 cells.
 
Creator Hemalatha, N.
Baskaran, V.
 
Subject 13 Molecular Biochemistry
04 Diabetes Mellitus
 
Description The stimulation of adenosine monophosphate‐activated protein kinase (AMPK) is a
prime target to decrease the hyperglycemic condition, hence it is a lutein (L) and
oxidised lutein (OXL) is a target molecule for the treatment of type II diabetes. In the
current study, a plausible interaction of L and OXL with AMPK was investigated by
molecular docking. In addition, the effect of L and OXL for the activation of AMPK
that triggers the downstream regulator peroxisome proliferator‐activated receptor γ
coactivator 1α (PGC‐1α), TFAM expression, mitochondrial DNA (mtDNA),
mitochondrial biogenesis and superoxide dismutase 2 (SOD2) in high glucose
treated HepG2 cells were investigated by quantitative polymerase chain reaction
and Western blot analysis. Molecular docking reveals higher binding affinity of
L (ΔG = −6.3 kcal/mol) and OXL (ΔG = −15.5 kcal/mol) with AMPK, compared
with metformin (ΔG = −5.0 kcal/mol). The phosphorylation of AMPK increased by
1.3‐ and 1.5‐fold with L and OXL treatment, respectively, in high glucose induced
HepG2 cells. The activation of PGC‐1α is significant (P < 0.05) in OXL group than
L. Similarly, TFAM expression is increased with L and OXL compared with the high
glucose group. Further increase in SOD2 and mtDNA, confirms the efficacy of L and
OXL in restoring the mitochondrial biogenesis in high glucose induced cells through
AMPK, PGC‐1α, and TFAM.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14631/1/J%20Cell%20Biochem.%202019%3B12015255-15267.pdf
Hemalatha, N. and Baskaran, V. (2019) Enhanced phosphorylation of AMPK by lutein and oxidised lutein that lead to mitochondrial biogenesis in hyperglycemic HepG2 cells. Journal of Cell Biochemistry, 120. pp. 15255-15267.