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Probiotic Yeasts Inhibit Virulence of Non-albicans Candida Species.

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Relation http://ir.cftri.com/14639/
 
Title Probiotic Yeasts Inhibit Virulence of Non-albicans Candida
Species.
 
Creator Lohith, K.
Kurrey, N. K.
Anu Appaiah, K. A.
Reeta, P. Rao
 
Subject 04 Yeast
 
Description Systemic infections of Candida species pose a significant threat to public health. Toxicity associated with current therapies and emergence of resistant
strains present major therapeutic challenges. Here, we report exploitation of the probiotic properties of two novel, food-derived yeasts, Saccharomyces cerevisiae (strain
KTP) and Issatchenkia occidentalis (strain ApC), as an alternative approach to combat
widespread opportunistic fungal infections. Both yeasts inhibit virulence traits such
as adhesion, filamentation, and biofilm formation of several non-albicans Candida
species, including Candida tropicalis, Candida krusei, Candida glabrata, and Candida
parapsilosis as well as the recently identified multidrug-resistant species Candida auris. They inhibit adhesion to abiotic surfaces as well as cultured colon epithelial cells.
Furthermore, probiotic treatment blocks the formation of biofilms of individual nonalbicans Candida strains as well as mixed-culture biofilms of each non-albicans Candida strain in combination with Candida albicans. The probiotic yeasts attenuated
non-albicans Candida infections in a live animal. In vivo studies using Caenorhabditis
elegans suggest that exposure to probiotic yeasts protects nematodes from infection
with non-albicans Candida strains compared to worms that were not exposed to the
probiotic yeasts. Furthermore, application of probiotic yeasts postinfection with nonalbicans Candida alleviated pathogenic colonization of the nematode gut. The probiotic properties of these novel yeasts are better than or comparable to those of the
commercially available probiotic yeast Saccharomyces boulardii, which was used as a
reference strain throughout this study. These results indicate that yeasts derived
from food sources could serve as an effective alternative to antifungal therapy
against emerging pathogenic Candida species.
IMPORTANCE Non-albicans Candida-associated infections have emerged as a major
risk factor in the hospitalized and immunecompromised patients. Besides, antifungalassociated complications occur more frequently with these non-albicans Candida
species than with C. albicans. Therefore, as an alternative approach to combat these
widespread non-albicans Candida-associated infections, here we showed the probiotic effect of two yeasts, Saccharomyces cerevisiae (strain KTP) and Issatchenkia occidentalis (ApC), in preventing adhesion and biofilm formation of five non-albicans
Candida strains, Candida tropicalis, Candida krusei, Candida glabrata, Candida parapsilosis, and Candida auris. The result would influence the current trend of the conversion of conventional antimicrobial therapy into beneficial probiotic microbeassociated antimicrobial treatment.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14639/1/mBio%202019.pdf
Lohith, K. and Kurrey, N. K. and Anu Appaiah, K. A. and Reeta, P. Rao (2019) Probiotic Yeasts Inhibit Virulence of Non-albicans Candida Species. mBio, 10 (5). e02307-19.