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Guggulipid ameliorates adjuvant-induced arthritis and liver oxidative damage by suppressing inflammatory and oxidative stress mediators.

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Relation http://ir.cftri.com/14643/
https://doi.org/10.1016/j.phymed.2019.152924
 
Title Guggulipid ameliorates adjuvant-induced arthritis and liver oxidative
damage by suppressing inflammatory and oxidative stress mediators.
 
Creator Sundaram, M. S.
Manoj Kumar, Neog
Mahaboobkhan, Rasool
Suresh Kumar, G.
 
Subject 10 Plants
11 Lipid Biochemistry
 
Description Background: Arthritis is a common degenerative joint disease characterized by deterioration of articular cartilage, subchondral bone, and associated with immobility, pain and inflammation. The incessant action of reactive
oxygen species (ROS) during progressive arthritis causes severe oxidative damage to vital organs and circulatory
system.
Purpose: In this study we investigated the ability of guggulipid (GL), a lipid rich extract from the gum resin of the
plant Commiphora whighitii to suppress the progressive arthritis and associated liver oxidative stress both in vivo
and in vitro.
Study design/Methods: The anti-arthritic ability of GL was demonstrated in vitro using IL-1β stimulated bovine
nasal cartilage model and in vivo Freund's complete adjuvant-induced arthritic rat model. Collagen/proteoglycan
degradation and pro-inflammatory mediators were monitored in the harvested culture medium of nasal cartilage
by estimating the levels of matrix metalloproteinases (MMPs), hydroxy proline, glycosaminoglycans and inflammatory mediators. Further, anti-arthritic ability of GL was evaluated in vivo by measuring enzymatic and
non-enzymatic mediators of cartilage degradation, inflammation and oxidative stress markers.
Results: GL significantly inhibited the IL-1β stimulated cartilage degradation in vitro by mitigating the MMPs
activity, collagen degradation and secretion of pro-inflammatory mediators. Further, GL significantly reduced
the adjuvant-induced paw swelling and body weight loss in vivo. GL remarkably reduced the MMPs and hyaluronidases activities in serum and bone homogenate along with altered hematological parameters. GL also
mitigated the elevated bone resorbing enzymes cathepsins, exoglycosidases and phosphatases. Additionally, GL
effectively mitigated ROS and oxidative stress-mediators recuperating the altered serum/liver oxidative stress
and liver damage incurred during arthritic progression.
Conclusion: In summary, the study clearly demonstrates the protective efficacy of GL against arthritis and its
associated oxidative stress, particularly, liver oxidative damage. Hence, GL could be a potential alternative and
complementary medicine to treat inflammatory joint diseases
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14643/1/Phytomedicine%2064%20%282019%29%20152924.pdf
Sundaram, M. S. and Manoj Kumar, Neog and Mahaboobkhan, Rasool and Suresh Kumar, G. (2019) Guggulipid ameliorates adjuvant-induced arthritis and liver oxidative damage by suppressing inflammatory and oxidative stress mediators. Phytomedicine, 64. p. 152924. ISSN 1618-095X