Record Details

Crosstalk between protein N-glycosylation and lipid metabolism in Saccharomyces cerevisiae.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/14646/
https://doi.org/10.1038/s41598-019-51054-7
 
Title Crosstalk between protein N-glycosylation and lipid
metabolism in Saccharomyces cerevisiae.
 
Creator Antonisamy, William James
Ravi, C.
Malathi, Srinivasan
Vasanthi, Nachiappan
 
Subject 11 Lipid Biochemistry
 
Description The endoplasmic reticulum (ER) is a multi functional organelle and plays a crucial role in protein folding
and lipid biosynthesis. The SEC59 gene encodes dolichol kinase, required for protein glycosylation in
the ER. The mutation of sec59-1 caused a protein N-glycosylation defect mediated ER stress resulting in
increased levels of phospholipid, neutral lipid and sterol, whereas growth was reduced. In the sec59-1∆
cell, the N-glycosylation of vacuolar carboxy peptidase-Y (CPY) was signifcantly reduced; whereas the
ER stress marker Kar2p and unfolded protein response (UPR) were signifcantly increased. Increased
levels of Triacylglycerol (TAG), sterol ester (SE), and lipid droplets (LD) could be attributed to upregulation of DPP1, LRO1, and ARE2 in the sec 59-1∆ cell. Also, the diacylglycerol (DAG), sterol (STE),
and free fatty acids (FFA) levels were signifcantly increased, whereas the genes involved in peroxisome
biogenesis and Pex3-EGFP levels were reduced when compared to the wild-type. The microarray
data also revealed increased expression of genes involved in phospholipid, TAG, fatty acid, sterol
synthesis, and phospholipid transport resulting in dysregulation of lipid homeostasis in the sec59-1∆
cell. We conclude that SEC59 dependent N-glycosylation is required for lipid homeostasis, peroxisome
biogenesis, and ER protein quality control.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14646/1/Scientific%20Reports%20%20%282019%29%20914485.pdf
Antonisamy, William James and Ravi, C. and Malathi, Srinivasan and Vasanthi, Nachiappan (2019) Crosstalk between protein N-glycosylation and lipid metabolism in Saccharomyces cerevisiae. Scientific Reports, 9. p. 14485.