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Inhibitory efficacy of lutein on adipogenesis is associated with blockage of early phase regulators of adipocyte differentiation.

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Relation http://ir.cftri.com/14650/
https://doi.org/10.1016/j.bbalip.2020.158812
 
Title Inhibitory efficacy of lutein on adipogenesis is associated with blockage of
early phase regulators of adipocyte differentiation.
 
Creator Sowmyashree, G.
Sachin, M. Eligar
Baskaran, V.
Ganesan, P.
 
Subject 10 Plants
14 Carotenoid Chemistry
04 Diabetes Mellitus
 
Description A comprehensive molecular mechanistic role of lutein on adipogenesis is not well understood. The present study
focused to evaluate the effect of lutein at the early and late phase of adipocyte differentiation in vitro using a
3T3-L1 cell model. The effect of purified carotenoid on the viability of normal and differentiated 3T3-L1 cells
was analyzed by WST-1 assay. Oil Red O and Nile red staining were employed to observe lipid droplets in mature
adipocytes. The effect of lutein on gene and protein expression of major transcription factors and adipogenic
markers was analyzed by RT-PCR and western blotting, respectively. The role of lutein on mitotic clonal expansion was analyzed by flow cytometry. The results showed a significant reduction (p < 0.05) in the accumulation of lipid droplets in lutein-treated (5 μM) cells. Inhibition in lipid accumulation was associated with
down-regulated expression of CEBP-α and PPAR-γ at gene and protein levels. Subsequently, lutein repressed
gene expression of FAS, FABP4, and SCD1 in mature adipocytes. Interestingly, it blocks the protein expression of
CEBP-α and PPAR-γ in the initial stages of adipocyte differentiation. This early-stage inhibition of adipocyte
differentiation is linked with repressed phosphorylation AKT and ERK. Further, upregulated cyclin D and downregulated CDK4 and CDK2 in lutein treated adipocytes enumerate its role in delaying the cell cycle progression at
the G0/G1 phase. Our results emphasize that adipogenesis inhibitory efficacy of lutein is potentiated by halting
early phase regulators of adipocyte differentiation, which strengthens the competency of lutein besides its inevitable presence in the human body.
 
Date 2021
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14650/1/BBA%20-%20Molecular%20and%20Cell%20Biology%20of%20Lipids%201866%20%282021%29%20158812.pdf
Sowmyashree, G. and Sachin, M. Eligar and Baskaran, V. and Ganesan, P. (2021) Inhibitory efficacy of lutein on adipogenesis is associated with blockage of early phase regulators of adipocyte differentiation. BBA - Molecular and Cell Biology of Lipids, 1866. p. 158812.