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Sequence characterization and N-glycoproteomics of secretory immunoglobulin A from donkey milk.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/14661/
https://doi.org/10.1016/j.ijbiomac.2020.03.253 0141-8130/
 
Title Sequence characterization and N-glycoproteomics of secretory
immunoglobulin A from donkey milk.
 
Creator Gnanesh Kumar, B. S.
Apoorva, Rawal
 
Subject 11 Animals
03 Proteins
27 Dairy products
 
Description Secretory immunoglobulin A (sIgA) is the major antibody present in the human milk where it confers passive immunity to neonates. Other than human, non-ruminants such as equine, swine etc., also possess sIgA in milk but
detailed characterization is limited. In the present study, we characterized sIgA from donkey milk for amino acid
sequence and N-glycosylation through LC-MS/MS analysis. The complete amino acid sequence of alpha chain
constant region (CH) was elucidated. The sequence analysis of variable regions (VH and VL) and light chain constant region (CL) showed several amino acid substitutions indicating the presence of diverse immunoglobulin
repertoire. Glycoproteomic analysis of secretory component revealed bi-antennary complex and hybrid types
with differential core fucosylation at site N83LT, only complex glycans at N135GT, N423GT and N530LT with mainly
NeuAc whereas N291QT harbors high mannose glycans. Heavy chain possesses majorly bi-antennary complex
with differential core fucosylation at sites N139AS and N338VS, in which N338VS shows partial occupancy. Joining
chain harbors only complex type at N72IS, with core fucosylation and terminal NeuGc to some extent. N-glycan
repertoire in part is similar to human sIgA. This comprehensive analysis of sequence and glycan pattern of donkey
milk sIgA would be beneficial for its potential applications.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14661/1/International%20Journal%20of%20Biological%20Macromolecules%20155%20%282020%29%20605%E2%80%93613.pdf
Gnanesh Kumar, B. S. and Apoorva, Rawal (2020) Sequence characterization and N-glycoproteomics of secretory immunoglobulin A from donkey milk. International Journal of Biological Macromolecules (155). pp. 605-613. ISSN 0141-8130